TY - JOUR
T1 - High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach
AU - Mollerup, Sarah
AU - Asplund, Maria
AU - Friis-Nielsen, Jens
AU - Kjartansdóttir, Kristín Rós
AU - Fridholm, Helena
AU - Hansen, Thomas Arn
AU - Herrera, José Alejandro Romero
AU - Barnes, Christopher James
AU - Jensen, Randi Holm
AU - Richter, Stine Raith
AU - Nielsen, Ida Broman
AU - Pietroni, Carlotta
AU - Alquezar-Planas, David E
AU - Rey-Iglesia, Alba
AU - Olsen, Pernille V S
AU - Rajpert-De Meyts, Ewa
AU - Groth-Pedersen, Line
AU - von Buchwald, Christian
AU - Jensen, David H
AU - Gniadecki, Robert
AU - Høgdall, Estrid
AU - Langhoff, Jill Levin
AU - Pete, Imre
AU - Vereczkey, Ildikó
AU - Baranyai, Zsolt
AU - Dybkaer, Karen
AU - Johnsen, Hans Erik
AU - Steiniche, Torben
AU - Hokland, Peter
AU - Rosenberg, Jacob
AU - Baandrup, Ulrik
AU - Sicheritz-Pontén, Thomas
AU - Willerslev, Eske
AU - Brunak, Søren
AU - Lund, Ole
AU - Mourier, Tobias
AU - Vinner, Lasse
AU - Izarzugaza, Jose M G
AU - Nielsen, Lars Peter
AU - Hansen, Anders Johannes
N1 - © The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America.
PY - 2019/10/15
Y1 - 2019/10/15
N2 - BACKGROUND: Viruses and other infectious agents cause more than 15% of human cancer cases. High-throughput sequencing-based studies of virus-cancer associations have mainly focused on cancer transcriptome data.METHODS: In this study, we applied a diverse selection of presequencing enrichment methods targeting all major viral groups, to characterize the viruses present in 197 samples from 18 sample types of cancerous origin. Using high-throughput sequencing, we generated 710 datasets constituting 57 billion sequencing reads.RESULTS: Detailed in silico investigation of the viral content, including exclusion of viral artefacts, from de novo assembled contigs and individual sequencing reads yielded a map of the viruses detected. Our data reveal a virome dominated by papillomaviruses, anelloviruses, herpesviruses, and parvoviruses. More than half of the included samples contained 1 or more viruses; however, no link between specific viruses and cancer types were found.CONCLUSIONS: Our study sheds light on viral presence in cancers and provides highly relevant virome data for future reference.
AB - BACKGROUND: Viruses and other infectious agents cause more than 15% of human cancer cases. High-throughput sequencing-based studies of virus-cancer associations have mainly focused on cancer transcriptome data.METHODS: In this study, we applied a diverse selection of presequencing enrichment methods targeting all major viral groups, to characterize the viruses present in 197 samples from 18 sample types of cancerous origin. Using high-throughput sequencing, we generated 710 datasets constituting 57 billion sequencing reads.RESULTS: Detailed in silico investigation of the viral content, including exclusion of viral artefacts, from de novo assembled contigs and individual sequencing reads yielded a map of the viruses detected. Our data reveal a virome dominated by papillomaviruses, anelloviruses, herpesviruses, and parvoviruses. More than half of the included samples contained 1 or more viruses; however, no link between specific viruses and cancer types were found.CONCLUSIONS: Our study sheds light on viral presence in cancers and provides highly relevant virome data for future reference.
U2 - 10.1093/infdis/jiz318
DO - 10.1093/infdis/jiz318
M3 - Journal article
C2 - 31253993
VL - 220
SP - 1312
EP - 1324
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 8
ER -