Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine

Lars Barregard; Peter Møller; Trine Henriksen; Vilas Mistry; Gudrun Koppen; Pavel Rossner; Radim J Sram; Allan Weimann; Henrik E Poulsen; Robert Nataf; Roberta Andreoli; Paola Manini; Tim Marczylo; Patricia Lam; Mark D Evans; Hiroshi Kasai; Kazuaki Kawai; Yun-Shan Li; Kazuo Sakai; Rajinder Singh; Friederike Teichert; Peter B Farmer; Rafal Rozalski; Daniel Gackowski; Agnieszka Siomek; Guillermo T Saez; Concha Cerda; Karin Broberg; Christian Lindh; Mohammad Bakhtiar Hossain; Siamak Haghdoost; Chiung-Wen Hu; Mu-Rong Chao; Kuen-Yuh Wu; Hilmi Orhan; Nilufer Senduran; Raymond J Smith; Regina M Santella; Yali Su; Czarina Cortez; Susan Yeh; Ryszard Olinski; Steffen Loft; Marcus S Cooke

doi:10.1089/ars.2012.4714

Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine

Lars Barregard, Peter Møller, Trine Henriksen, Vilas Mistry, Gudrun Koppen, Pavel Rossner, Radim J Sram, Allan Weimann, Henrik E Poulsen, Robert Nataf, Roberta Andreoli, Paola Manini, Tim Marczylo, Patricia Lam, Mark D Evans, Hiroshi Kasai, Kazuaki Kawai, Yun-Shan Li, Kazuo Sakai, Rajinder SinghFriederike Teichert, Peter B Farmer, Rafal Rozalski, Daniel Gackowski, Agnieszka Siomek, Guillermo T Saez, Concha Cerda, Karin Broberg, Christian Lindh, Mohammad Bakhtiar Hossain, Siamak Haghdoost, Chiung-Wen Hu, Mu-Rong Chao, Kuen-Yuh Wu, Hilmi Orhan, Nilufer Senduran, Raymond J Smith, Regina M Santella, Yali Su, Czarina Cortez, Susan Yeh, Ryszard Olinski, Steffen Loft, Marcus S Cooke

Research output: Contribution to journal › Journal article › Research › peer-review

128 Citations (Scopus)

Abstract

Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability.

Original language	English
Journal	Antioxidants & Redox Signaling
Volume	18
Issue number	18
Pages (from-to)	2377-91
Number of pages	15
ISSN	1523-0864
DOIs	https://doi.org/10.1089/ars.2012.4714
Publication status	Published - 20 Jun 2013

Keywords

Adult
Artifacts
Buffers
Deoxyguanosine
Female
Head and Neck Neoplasms
Humans
Male
Middle Aged
Reference Standards
Reproducibility of Results
Sodium Chloride
Solutions
Urinalysis
Young Adult

Access to Document

10.1089/ars.2012.4714

Cite this

Barregard, L., Møller, P., Henriksen, T., Mistry, V., Koppen, G., Rossner, P., Sram, R. J., Weimann, A., Poulsen, H. E., Nataf, R., Andreoli, R., Manini, P., Marczylo, T., Lam, P., Evans, M. D., Kasai, H., Kawai, K., Li, Y-S., Sakai, K., ... Cooke, M. S. (2013). Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine. Antioxidants & Redox Signaling, 18(18), 2377-91. https://doi.org/10.1089/ars.2012.4714

Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine. / Barregard, Lars; Møller, Peter; Henriksen, Trine; Mistry, Vilas; Koppen, Gudrun; Rossner, Pavel; Sram, Radim J; Weimann, Allan; Poulsen, Henrik E; Nataf, Robert; Andreoli, Roberta; Manini, Paola; Marczylo, Tim; Lam, Patricia; Evans, Mark D; Kasai, Hiroshi; Kawai, Kazuaki; Li, Yun-Shan; Sakai, Kazuo; Singh, Rajinder; Teichert, Friederike; Farmer, Peter B; Rozalski, Rafal; Gackowski, Daniel; Siomek, Agnieszka; Saez, Guillermo T; Cerda, Concha; Broberg, Karin; Lindh, Christian; Hossain, Mohammad Bakhtiar; Haghdoost, Siamak; Hu, Chiung-Wen; Chao, Mu-Rong; Wu, Kuen-Yuh; Orhan, Hilmi; Senduran, Nilufer; Smith, Raymond J; Santella, Regina M; Su, Yali; Cortez, Czarina; Yeh, Susan; Olinski, Ryszard; Loft, Steffen; Cooke, Marcus S.

In: Antioxidants & Redox Signaling, Vol. 18, No. 18, 20.06.2013, p. 2377-91.

Research output: Contribution to journal › Journal article › Research › peer-review

Barregard, L, Møller, P, Henriksen, T, Mistry, V, Koppen, G, Rossner, P, Sram, RJ, Weimann, A, Poulsen, HE, Nataf, R, Andreoli, R, Manini, P, Marczylo, T, Lam, P, Evans, MD, Kasai, H, Kawai, K, Li, Y-S, Sakai, K, Singh, R, Teichert, F, Farmer, PB, Rozalski, R, Gackowski, D, Siomek, A, Saez, GT, Cerda, C, Broberg, K, Lindh, C, Hossain, MB, Haghdoost, S, Hu, C-W, Chao, M-R, Wu, K-Y, Orhan, H, Senduran, N, Smith, RJ, Santella, RM, Su, Y, Cortez, C, Yeh, S, Olinski, R, Loft, S & Cooke, MS 2013, 'Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine', Antioxidants & Redox Signaling, vol. 18, no. 18, pp. 2377-91. https://doi.org/10.1089/ars.2012.4714

Barregard, Lars ; Møller, Peter ; Henriksen, Trine ; Mistry, Vilas ; Koppen, Gudrun ; Rossner, Pavel ; Sram, Radim J ; Weimann, Allan ; Poulsen, Henrik E ; Nataf, Robert ; Andreoli, Roberta ; Manini, Paola ; Marczylo, Tim ; Lam, Patricia ; Evans, Mark D ; Kasai, Hiroshi ; Kawai, Kazuaki ; Li, Yun-Shan ; Sakai, Kazuo ; Singh, Rajinder ; Teichert, Friederike ; Farmer, Peter B ; Rozalski, Rafal ; Gackowski, Daniel ; Siomek, Agnieszka ; Saez, Guillermo T ; Cerda, Concha ; Broberg, Karin ; Lindh, Christian ; Hossain, Mohammad Bakhtiar ; Haghdoost, Siamak ; Hu, Chiung-Wen ; Chao, Mu-Rong ; Wu, Kuen-Yuh ; Orhan, Hilmi ; Senduran, Nilufer ; Smith, Raymond J ; Santella, Regina M ; Su, Yali ; Cortez, Czarina ; Yeh, Susan ; Olinski, Ryszard ; Loft, Steffen ; Cooke, Marcus S. / Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine. In: Antioxidants & Redox Signaling. 2013 ; Vol. 18, No. 18. pp. 2377-91.

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title = "Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine",

abstract = "Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability.",

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AU - Mistry, Vilas

AU - Koppen, Gudrun

AU - Rossner, Pavel

AU - Sram, Radim J

AU - Weimann, Allan

AU - Poulsen, Henrik E

AU - Nataf, Robert

AU - Andreoli, Roberta

AU - Manini, Paola

AU - Marczylo, Tim

AU - Lam, Patricia

AU - Evans, Mark D

AU - Kasai, Hiroshi

AU - Kawai, Kazuaki

AU - Li, Yun-Shan

AU - Sakai, Kazuo

AU - Singh, Rajinder

AU - Teichert, Friederike

AU - Farmer, Peter B

AU - Rozalski, Rafal

AU - Gackowski, Daniel

AU - Siomek, Agnieszka

AU - Saez, Guillermo T

AU - Cerda, Concha

AU - Broberg, Karin

AU - Lindh, Christian

AU - Hossain, Mohammad Bakhtiar

AU - Haghdoost, Siamak

AU - Hu, Chiung-Wen

AU - Chao, Mu-Rong

AU - Wu, Kuen-Yuh

AU - Orhan, Hilmi

AU - Senduran, Nilufer

AU - Smith, Raymond J

AU - Santella, Regina M

AU - Su, Yali

AU - Cortez, Czarina

AU - Yeh, Susan

AU - Olinski, Ryszard

AU - Loft, Steffen

AU - Cooke, Marcus S

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KW - Buffers

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KW - Female

KW - Head and Neck Neoplasms

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KW - Male

KW - Middle Aged

KW - Reference Standards

KW - Reproducibility of Results

KW - Sodium Chloride

KW - Solutions

KW - Urinalysis

KW - Young Adult

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