Kalanchoë blossfeldiana naturally transformed with Rhizobium rhizogenes exhibits superior root phenotype

Bruno Trevenzoli Favero; Yi Tan; Xuefei Chen; Renate Müller; Henrik Lütken

doi:10.1016/j.plantsci.2022.111323

Kalanchoë blossfeldiana naturally transformed with Rhizobium rhizogenes exhibits superior root phenotype

Bruno Trevenzoli Favero^*, Yi Tan, Xuefei Chen, Renate Müller, Henrik Lütken

^*Corresponding author for this work

Section for Crop Sciences

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Plant transformation with root oncogenic loci (rol) genes and open reading frames (ORFs) from Rhizobium rhizogenes have not yet targeted the underground root phenotype of these transformants. Hence, there is a need to develop plants with more efficient root system architecture (RSA). Here, RSA was assessed in naturally transformed (NT) and single rol/ORF Kalanchoë blossfeldiana 'Molly' lines in an aeroponic growth system combined with gene expression analysis. Three NT lines; 306, 324 and 331; exhibited better-developed RSA with longer roots and increased root biomass. In line 306, longest root was 6.3 ± 0.3 cm while WT had 4.8 ± 0.1 cm. However, root length of all overexpressing lines was ca. 30% shorter than WT. Root fresh weight of NT lines was 4.5-fold higher than WT. The expression of rolB, ∆ORF13a and ORF14 in the leaves of overexpressing lines was many folds higher than in NT lines. Increased expression of ∆ORF13a and ORF14 in leaves and roots may contribute more to a stronger compact phenotype than previously assumed. The moderate compact phenotype of NT lines combined with improved RSA compared to the overexpressing lines and WT strongly indicate that the use of R. rhizogenes has great potential to produce Kalanchoë phenotypes with enhanced RSA.

Original language	English
Article number	111323
Journal	Plant Science
Volume	321
Number of pages	10
ISSN	0168-9452
DOIs	https://doi.org/10.1016/j.plantsci.2022.111323 https://doi.org/10.1016/j.plantsci.2022.111323
Publication status	Published - 2022

Bibliographical note

Funding Information:
The authors thank Dr Josefine Nymark Hegelund and Shenglan Li for their guidance in performing qPCR. We also thank Yan Lin, Jiaming Xu and Junou He for their help and assistance in performing the biometric measurement. This research was supported by the Danish Council for Independent Research (Grant no. DFF-7017–00197 ). Yi Tan was financially supported by The Chinese Scholarship Council (Grant n. 201806300008 ). Xuefei Chen was financially supported by The Chinese Scholarship Council (Grant n. 202106850009 ).

Publisher Copyright:
© 2022 The Authors

Keywords

Gene expression
Root length density
root oncogenic loci
Root system architecture

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Cite this

@article{713977e0454c4444a0e37f760051f976,

title = "Kalancho{\"e} blossfeldiana naturally transformed with Rhizobium rhizogenes exhibits superior root phenotype",

abstract = "Plant transformation with root oncogenic loci (rol) genes and open reading frames (ORFs) from Rhizobium rhizogenes have not yet targeted the underground root phenotype of these transformants. Hence, there is a need to develop plants with more efficient root system architecture (RSA). Here, RSA was assessed in naturally transformed (NT) and single rol/ORF Kalancho{\"e} blossfeldiana 'Molly' lines in an aeroponic growth system combined with gene expression analysis. Three NT lines; 306, 324 and 331; exhibited better-developed RSA with longer roots and increased root biomass. In line 306, longest root was 6.3 ± 0.3 cm while WT had 4.8 ± 0.1 cm. However, root length of all overexpressing lines was ca. 30% shorter than WT. Root fresh weight of NT lines was 4.5-fold higher than WT. The expression of rolB, ∆ORF13a and ORF14 in the leaves of overexpressing lines was many folds higher than in NT lines. Increased expression of ∆ORF13a and ORF14 in leaves and roots may contribute more to a stronger compact phenotype than previously assumed. The moderate compact phenotype of NT lines combined with improved RSA compared to the overexpressing lines and WT strongly indicate that the use of R. rhizogenes has great potential to produce Kalancho{\"e} phenotypes with enhanced RSA.",

keywords = "Gene expression, Root length density, root oncogenic loci, Root system architecture",

author = "Favero, {Bruno Trevenzoli} and Yi Tan and Xuefei Chen and Renate M{\"u}ller and Henrik L{\"u}tken",

note = "Funding Information: The authors thank Dr Josefine Nymark Hegelund and Shenglan Li for their guidance in performing qPCR. We also thank Yan Lin, Jiaming Xu and Junou He for their help and assistance in performing the biometric measurement. This research was supported by the Danish Council for Independent Research (Grant no. DFF-7017–00197 ). Yi Tan was financially supported by The Chinese Scholarship Council (Grant n. 201806300008 ). Xuefei Chen was financially supported by The Chinese Scholarship Council (Grant n. 202106850009 ). Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

doi = "10.1016/j.plantsci.2022.111323",

language = "English",

volume = "321",

journal = "Plant Science",

issn = "0168-9452",

publisher = "Elsevier Ireland Ltd",

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TY - JOUR

T1 - Kalanchoë blossfeldiana naturally transformed with Rhizobium rhizogenes exhibits superior root phenotype

AU - Favero, Bruno Trevenzoli

AU - Tan, Yi

AU - Chen, Xuefei

AU - Müller, Renate

AU - Lütken, Henrik

N1 - Funding Information: The authors thank Dr Josefine Nymark Hegelund and Shenglan Li for their guidance in performing qPCR. We also thank Yan Lin, Jiaming Xu and Junou He for their help and assistance in performing the biometric measurement. This research was supported by the Danish Council for Independent Research (Grant no. DFF-7017–00197 ). Yi Tan was financially supported by The Chinese Scholarship Council (Grant n. 201806300008 ). Xuefei Chen was financially supported by The Chinese Scholarship Council (Grant n. 202106850009 ). Publisher Copyright: © 2022 The Authors

PY - 2022

Y1 - 2022

N2 - Plant transformation with root oncogenic loci (rol) genes and open reading frames (ORFs) from Rhizobium rhizogenes have not yet targeted the underground root phenotype of these transformants. Hence, there is a need to develop plants with more efficient root system architecture (RSA). Here, RSA was assessed in naturally transformed (NT) and single rol/ORF Kalanchoë blossfeldiana 'Molly' lines in an aeroponic growth system combined with gene expression analysis. Three NT lines; 306, 324 and 331; exhibited better-developed RSA with longer roots and increased root biomass. In line 306, longest root was 6.3 ± 0.3 cm while WT had 4.8 ± 0.1 cm. However, root length of all overexpressing lines was ca. 30% shorter than WT. Root fresh weight of NT lines was 4.5-fold higher than WT. The expression of rolB, ∆ORF13a and ORF14 in the leaves of overexpressing lines was many folds higher than in NT lines. Increased expression of ∆ORF13a and ORF14 in leaves and roots may contribute more to a stronger compact phenotype than previously assumed. The moderate compact phenotype of NT lines combined with improved RSA compared to the overexpressing lines and WT strongly indicate that the use of R. rhizogenes has great potential to produce Kalanchoë phenotypes with enhanced RSA.

AB - Plant transformation with root oncogenic loci (rol) genes and open reading frames (ORFs) from Rhizobium rhizogenes have not yet targeted the underground root phenotype of these transformants. Hence, there is a need to develop plants with more efficient root system architecture (RSA). Here, RSA was assessed in naturally transformed (NT) and single rol/ORF Kalanchoë blossfeldiana 'Molly' lines in an aeroponic growth system combined with gene expression analysis. Three NT lines; 306, 324 and 331; exhibited better-developed RSA with longer roots and increased root biomass. In line 306, longest root was 6.3 ± 0.3 cm while WT had 4.8 ± 0.1 cm. However, root length of all overexpressing lines was ca. 30% shorter than WT. Root fresh weight of NT lines was 4.5-fold higher than WT. The expression of rolB, ∆ORF13a and ORF14 in the leaves of overexpressing lines was many folds higher than in NT lines. Increased expression of ∆ORF13a and ORF14 in leaves and roots may contribute more to a stronger compact phenotype than previously assumed. The moderate compact phenotype of NT lines combined with improved RSA compared to the overexpressing lines and WT strongly indicate that the use of R. rhizogenes has great potential to produce Kalanchoë phenotypes with enhanced RSA.

KW - Gene expression

KW - Root length density

KW - root oncogenic loci

KW - Root system architecture

U2 - 10.1016/j.plantsci.2022.111323

DO - 10.1016/j.plantsci.2022.111323

M3 - Journal article

C2 - 35696923

SN - 0168-9452

VL - 321

JO - Plant Science

JF - Plant Science

M1 - 111323

ER -