Abstract
In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a P. aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.
Original language | English |
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Journal | Methods in Molecular Biology |
Volume | 692 |
Issue number | 2 |
Pages (from-to) | 147-157 |
Number of pages | 11 |
ISSN | 1064-3745 |
DOIs | |
Publication status | Published - 1 Jan 2011 |