Immunomagnetic Selection of Plasmodium falciparum–Infected Erythrocytes Expressing Particular PfEMP1 Variants

Mary Lopez-Perez; Rebecca Wendelboe Olsen

doi:10.1007/978-1-0716-2189-9_7

Immunomagnetic Selection of Plasmodium falciparum–Infected Erythrocytes Expressing Particular PfEMP1 Variants

Mary Lopez-Perez^*, Rebecca Wendelboe Olsen

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

3 Citations (Scopus)

Abstract

Plasmodium falciparum expresses a broad range of proteins on the surface of infected erythrocytes (IEs), including members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1 protein. The expression of the corresponding PfEMP1 is later tested by flow cytometry, and the selected parasites can be used for further analysis.

Original language	English
Title of host publication	Methods in Molecular Biology : Targeting the Surface of Infected Erythrocytes
Number of pages	10
Publisher	Humana Press
Publication date	2022
Pages	69-78
ISBN (Electronic)	978-1-0716-2189-9
DOIs	https://doi.org/10.1007/978-1-0716-2189-9_7
Publication status	Published - 2022

Series	Methods in Molecular Biology
Volume	2470
ISSN	1064-3745

Bibliographical note

Funding Information:
The authors’ research work is supported by the Danish International Development Agency—Danida (17-02-KU). Maiken Visti and Mette Madsen are thanked for their technical assistance. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Antibodies
Antiserum
Flow cytometry
Immunomagnetic selection
Magnetic beads
PfEMP1

Access to Document

10.1007/978-1-0716-2189-9_7

Cite this

Immunomagnetic Selection of Plasmodium falciparum–Infected Erythrocytes Expressing Particular PfEMP1 Variants. / Lopez-Perez, Mary ; Olsen, Rebecca Wendelboe.

Methods in Molecular Biology: Targeting the Surface of Infected Erythrocytes. Humana Press, 2022. p. 69-78 (Methods in Molecular Biology, Vol. 2470).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

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