Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops

Olivia Mullertz; Anne Hedengran; Zaynab Ahmad Mouhammad; Josefine Freiberg; Richard Nagymihaly; Jette Jacobsen; Susan Weng Larsen; Jeffrey Bair; Tor Utheim; Darlene A. Dartt; Steffen Heegaard; Goran Petrovski; Miriam Kolko

doi:10.1136/bmjophth-2021-000892

Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops

Olivia Mullertz, Anne Hedengran, Zaynab Ahmad Mouhammad, Josefine Freiberg, Richard Nagymihaly, Jette Jacobsen, Susan Weng Larsen, Jeffrey Bair, Tor Utheim, Darlene A. Dartt, Steffen Heegaard, Goran Petrovski, Miriam Kolko^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

12 Citations (Scopus)

44 Downloads (Pure)

Abstract

Objective To investigate the short-term impact on human conjunctival goblet cell (GC) survival and mucin release of acute exposure to benzalkonium chloride (BAK) preserved and preservative-free (PF) 0.005% (w/v) latanoprost (LT) eye drops, and to compare the eye drops' physicochemical properties.

Methods and analysis Primary GC cultures were established from human conjunctival donor tissue. The impact of eye drops on GC survival was assessed using a lactate dehydrogenase assay. Mucin release was evaluated through mucin-specific immunostaining. pH value, osmolality, drop mass and surface tension for all LT eye drops were measured.

Results After application with PF-LT for 30 min (min), the GC survival was maintained compared with control (p=0.9941), while all BAK-LT eye drops reduced survival with approximately 30% (p

Conclusion PF-LT compared with various branded and generic LT preparations containing BAK are less cytotoxic when applied to cultured GCs.

Original language	English
Article number	000892
Journal	BMJ Open Ophthalmology
Volume	6
Issue number	1
Number of pages	7
ISSN	2397-3269
DOIs	https://doi.org/10.1136/bmjophth-2021-000892
Publication status	Published - 2021

Keywords

TEAR FILM STABILITY
OPEN-ANGLE GLAUCOMA
BRANDED LATANOPROST
OCULAR HYPERTENSION
PHYSICAL-PROPERTIES
THERAPY
SURFACE
VIVO

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Mullertz, O., Hedengran, A., Mouhammad, Z. A., Freiberg, J., Nagymihaly, R., Jacobsen, J., Larsen, S. W., Bair, J., Utheim, T., Dartt, D. A., Heegaard, S., Petrovski, G., & Kolko, M. (2021). Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops. BMJ Open Ophthalmology, 6(1), [000892]. https://doi.org/10.1136/bmjophth-2021-000892

Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops. / Mullertz, Olivia; Hedengran, Anne ; Mouhammad, Zaynab Ahmad ; Freiberg, Josefine; Nagymihaly, Richard; Jacobsen, Jette ; Larsen, Susan Weng; Bair, Jeffrey; Utheim, Tor; Dartt, Darlene A.; Heegaard, Steffen; Petrovski, Goran; Kolko, Miriam.

In: BMJ Open Ophthalmology, Vol. 6, No. 1, 000892, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Mullertz, O, Hedengran, A , Mouhammad, ZA , Freiberg, J, Nagymihaly, R, Jacobsen, J , Larsen, SW, Bair, J, Utheim, T, Dartt, DA, Heegaard, S, Petrovski, G & Kolko, M 2021, 'Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops', BMJ Open Ophthalmology, vol. 6, no. 1, 000892. https://doi.org/10.1136/bmjophth-2021-000892

Mullertz O, Hedengran A , Mouhammad ZA , Freiberg J, Nagymihaly R, Jacobsen J et al. Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops. BMJ Open Ophthalmology. 2021;6(1). 000892. https://doi.org/10.1136/bmjophth-2021-000892

Mullertz, Olivia ; Hedengran, Anne ; Mouhammad, Zaynab Ahmad ; Freiberg, Josefine ; Nagymihaly, Richard ; Jacobsen, Jette ; Larsen, Susan Weng ; Bair, Jeffrey ; Utheim, Tor ; Dartt, Darlene A. ; Heegaard, Steffen ; Petrovski, Goran ; Kolko, Miriam. / Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops. In: BMJ Open Ophthalmology. 2021 ; Vol. 6, No. 1.

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title = "Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops",

abstract = "Objective To investigate the short-term impact on human conjunctival goblet cell (GC) survival and mucin release of acute exposure to benzalkonium chloride (BAK) preserved and preservative-free (PF) 0.005% (w/v) latanoprost (LT) eye drops, and to compare the eye drops' physicochemical properties.Methods and analysis Primary GC cultures were established from human conjunctival donor tissue. The impact of eye drops on GC survival was assessed using a lactate dehydrogenase assay. Mucin release was evaluated through mucin-specific immunostaining. pH value, osmolality, drop mass and surface tension for all LT eye drops were measured.Results After application with PF-LT for 30 min (min), the GC survival was maintained compared with control (p=0.9941), while all BAK-LT eye drops reduced survival with approximately 30% (pConclusion PF-LT compared with various branded and generic LT preparations containing BAK are less cytotoxic when applied to cultured GCs.",

keywords = "TEAR FILM STABILITY, OPEN-ANGLE GLAUCOMA, BRANDED LATANOPROST, OCULAR HYPERTENSION, PHYSICAL-PROPERTIES, THERAPY, SURFACE, VIVO",

author = "Olivia Mullertz and Anne Hedengran and Mouhammad, {Zaynab Ahmad} and Josefine Freiberg and Richard Nagymihaly and Jette Jacobsen and Larsen, {Susan Weng} and Jeffrey Bair and Tor Utheim and Dartt, {Darlene A.} and Steffen Heegaard and Goran Petrovski and Miriam Kolko",

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AU - Mullertz, Olivia

AU - Hedengran, Anne

AU - Mouhammad, Zaynab Ahmad

AU - Freiberg, Josefine

AU - Nagymihaly, Richard

AU - Jacobsen, Jette

AU - Larsen, Susan Weng

AU - Bair, Jeffrey

AU - Utheim, Tor

AU - Dartt, Darlene A.

AU - Heegaard, Steffen

AU - Petrovski, Goran

AU - Kolko, Miriam

PY - 2021

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N2 - Objective To investigate the short-term impact on human conjunctival goblet cell (GC) survival and mucin release of acute exposure to benzalkonium chloride (BAK) preserved and preservative-free (PF) 0.005% (w/v) latanoprost (LT) eye drops, and to compare the eye drops' physicochemical properties.Methods and analysis Primary GC cultures were established from human conjunctival donor tissue. The impact of eye drops on GC survival was assessed using a lactate dehydrogenase assay. Mucin release was evaluated through mucin-specific immunostaining. pH value, osmolality, drop mass and surface tension for all LT eye drops were measured.Results After application with PF-LT for 30 min (min), the GC survival was maintained compared with control (p=0.9941), while all BAK-LT eye drops reduced survival with approximately 30% (pConclusion PF-LT compared with various branded and generic LT preparations containing BAK are less cytotoxic when applied to cultured GCs.

AB - Objective To investigate the short-term impact on human conjunctival goblet cell (GC) survival and mucin release of acute exposure to benzalkonium chloride (BAK) preserved and preservative-free (PF) 0.005% (w/v) latanoprost (LT) eye drops, and to compare the eye drops' physicochemical properties.Methods and analysis Primary GC cultures were established from human conjunctival donor tissue. The impact of eye drops on GC survival was assessed using a lactate dehydrogenase assay. Mucin release was evaluated through mucin-specific immunostaining. pH value, osmolality, drop mass and surface tension for all LT eye drops were measured.Results After application with PF-LT for 30 min (min), the GC survival was maintained compared with control (p=0.9941), while all BAK-LT eye drops reduced survival with approximately 30% (pConclusion PF-LT compared with various branded and generic LT preparations containing BAK are less cytotoxic when applied to cultured GCs.

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