Impaired Vitamin D Signaling in T Cells From a Family With Hereditary Vitamin D Resistant Rickets

Fatima A. H. Al-Jaberi, Martin Kongsbak-Wismann, Alejandro Aguayo-Orozco, Nicolai Krogh, Terkild B. Buus, Daniel V. Lopez, Anna K. O. Rode, Eva Gravesen, Klaus Olgaard, Soren Brunak, Anders Woetmann, Niels odum, Charlotte M. Bonefeld, Carsten Geisler*

*Corresponding author for this work

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Abstract

The active form of vitamin D, 1,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3), mediates its immunomodulatory effects by binding to the vitamin D receptor (VDR). Here, we describe a new point mutation in the DNA-binding domain of the VDR and its consequences for 1,25(OH)(2)D-3 signaling in T cells from heterozygous and homozygous carriers of the mutation. The mutation did not affect the overall structure or the ability of the VDR to bind 1,25(OH)(2)D-3 and the retinoid X receptor. However, the subcellular localization of the VDR was strongly affected and the transcriptional activity was abolished by the mutation. In heterozygous carriers of the mutation, 1,25(OH)(2)D-3-induced gene regulation was reduced by similar to 50% indicating that the expression level of wild-type VDR determines 1,25(OH)(2)D-3 responsiveness in T cells. We show that vitamin D-mediated suppression of vitamin A-induced gene regulation depends on an intact ability of the VDR to bind DNA. Furthermore, we demonstrate that vitamin A inhibits 1,25(OH)(2)D-3-induced translocation of the VDR to the nucleus and 1,25(OH)(2)D-3-induced up-regulation of CYP24A1. Taken together, this study unravels novel aspects of vitamin D signaling and function of the VDR in human T cells.

Original languageEnglish
Article number684015
JournalFrontiers in Immunology
Volume12
Number of pages11
ISSN1664-3224
DOIs
Publication statusPublished - 2021

Keywords

  • vitamin D
  • vitamin D receptor
  • HVDRR
  • T cells
  • vitamin A
  • D-RECEPTOR
  • DOWN-REGULATION
  • ACID

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