Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts

Bent Larsen Petersen; Svenning Rune Möller; Jozef Mravec; Bodil Jørgensen; Mikkel Christensen; Ying Liu; Hans H. Wandall; Eric Paul Bennett; Zhang Yang

doi:10.1186/s12896-019-0530-x

Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts

Bent Larsen Petersen, Svenning Rune Möller, Jozef Mravec, Bodil Jørgensen, Mikkel Christensen, Ying Liu, Hans H. Wandall, Eric Paul Bennett, Zhang Yang

Research output: Contribution to journal › Journal article › Research › peer-review

22 Citations (Scopus)

411 Downloads (Pure)

Original language	English
Article number	36
Journal	BMC Biotechnology
Volume	19
Pages (from-to)	1-12
ISSN	1472-6750
DOIs	https://doi.org/10.1186/s12896-019-0530-x
Publication status	Published - 2019

Keywords

Precise genetic editing
Genome engineering
CRISPR
Cas9
Protoplasting
Fluorescence activated cell sorting
Mutation enrichment
Nicotiana benthamiana

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10.1186/s12896-019-0530-xLicence: CC BY

Improved CRISPRCas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplastsFinal published version, 2.08 MBLicence: CC BY

Cite this

Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts. / Petersen, Bent Larsen; Möller, Svenning Rune; Mravec, Jozef ; Jørgensen, Bodil; Christensen, Mikkel; Liu, Ying; Wandall, Hans H.; Bennett, Eric Paul ; Yang, Zhang.

In: BMC Biotechnology, Vol. 19, 36, 2019, p. 1-12.

Research output: Contribution to journal › Journal article › Research › peer-review

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AU - Christensen, Mikkel

AU - Liu, Ying

AU - Wandall, Hans H.

AU - Bennett, Eric Paul

AU - Yang, Zhang

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KW - Cas9

KW - Protoplasting

KW - Fluorescence activated cell sorting

KW - Mutation enrichment

KW - Nicotiana benthamiana

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