Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells

Thomas Gadegaard Koch; Preben Dybdahl Thomsen; Dean H. Betts

doi:10.1080/14653240902887259

Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells

Thomas Gadegaard Koch
, Preben Dybdahl Thomsen
, Dean H. Betts

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

BACKGROUND AIMS: A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs. METHODS: Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. RESULTS AND CONCLUSIONS: Our results indicate that PrepaCyte-EQ medium is superior to Ficoll-Paque PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.

Original language	English
Journal	Cytotherapy
Volume	11
Issue number	4
Pages (from-to)	443-447
Number of pages	5
ISSN	1465-3249
DOIs	https://doi.org/10.1080/14653240902887259
Publication status	Published - 2009

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title = "Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells",

abstract = " BACKGROUND AIMS: A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs. METHODS: Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. RESULTS AND CONCLUSIONS: Our results indicate that PrepaCyte-EQ medium is superior to Ficoll-Paque PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.",

author = "Koch, \{Thomas Gadegaard\} and Thomsen, \{Preben Dybdahl\} and Betts, \{Dean H.\}",

year = "2009",

doi = "10.1080/14653240902887259",

language = "English",

volume = "11",

pages = "443--447",

journal = "Cytotherapy",

issn = "1465-3249",

publisher = "Elsevier",

number = "4",

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TY - JOUR

T1 - Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells

AU - Koch, Thomas Gadegaard

AU - Thomsen, Preben Dybdahl

AU - Betts, Dean H.

PY - 2009

Y1 - 2009

N2 - BACKGROUND AIMS: A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs. METHODS: Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. RESULTS AND CONCLUSIONS: Our results indicate that PrepaCyte-EQ medium is superior to Ficoll-Paque PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.

AB - BACKGROUND AIMS: A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs. METHODS: Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. RESULTS AND CONCLUSIONS: Our results indicate that PrepaCyte-EQ medium is superior to Ficoll-Paque PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.

U2 - 10.1080/14653240902887259

DO - 10.1080/14653240902887259

M3 - Journal article

C2 - 19513899

SN - 1465-3249

VL - 11

SP - 443

EP - 447

JO - Cytotherapy

JF - Cytotherapy

IS - 4

ER -