TY - JOUR
T1 - Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine
AU - Kristensen, Mette
AU - Krogholm, Kirstine S.
AU - Frederiksen, Hanne
AU - Duus, Fritz
AU - Cornett, Claus
AU - Bügel, Susanne Gjedsted
AU - Rasmussen, Salka E.
PY - 2007
Y1 - 2007
N2 - A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesisof the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-l-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1±25.2% of the dose.
AB - A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesisof the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-l-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1±25.2% of the dose.
KW - Former LIFE faculty
KW - Isothiocyantes
KW - Biomarker
KW - Synthesis
KW - 1,3-Benzodithiol e-2-thione
KW - N-acetyl-S(N4-methylsulfinylbutythihocarbam oyl)4-cystione
U2 - 10.1016/j.jchromb.2007.01.022
DO - 10.1016/j.jchromb.2007.01.022
M3 - Journal article
C2 - 17307405
VL - 852
SP - 229
EP - 234
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 1-2
ER -