Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine

Mette Kristensen, Kirstine S. Krogholm, Hanne Frederiksen, Fritz Duus, Claus Cornett, Susanne Gjedsted Bügel, Salka E. Rasmussen

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    12 Citations (Scopus)

    Abstract

    A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesisof the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-l-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1±25.2% of the dose.

    Original languageEnglish
    JournalJournal of Chromatography - B
    Volume852
    Issue number1-2
    Pages (from-to)229-234
    Number of pages6
    ISSN1570-0232
    DOIs
    Publication statusPublished - 2007

    Keywords

    • Former LIFE faculty
    • Isothiocyantes
    • Biomarker
    • Synthesis
    • 1,3-Benzodithiol e-2-thione
    • N-acetyl-S(N4-methylsulfinylbutythihocarbam oyl)4-cystione

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