In situ atomic force microscope imaging of supported lipid bilayers

Thomas Kaasgaard; Chad Leidy; John Hjort Ipsen; Ole G. Mouritsen; Kent Jørgensen

doi:10.1002/1438-5171(200107)2:2<105::AID-SIMO105>3.0.CO;2-0

In situ atomic force microscope imaging of supported lipid bilayers

Thomas Kaasgaard, Chad Leidy, John Hjort Ipsen, Ole G. Mouritsen, Kent Jørgensen

Research output: Contribution to journal › Journal article › Research › peer-review

33 Citations (Scopus)

Abstract

In situ AFM images of phospholipase A₂ (PLA₂) hydrolysis of mica-supported one- and two-component lipid bilayers are presented. For one-component DPPC bilayers an enhanced enzymatic activity is observed towards preexisting defects in the bilayer. Phase separation is observed in two-component DMPC-DSPC bilayers and a remarkable enhanced hydrolytic activity of the PLA₂-enzyme for the DMPC-rich phase is seen. Furthermore, in a supported double bilayer system a characteristic ripple structure, most likely related to the formation of the P_β'-ripple phase is observed.

Original language	English
Journal	Single Molecules
Volume	2
Issue number	2
Pages (from-to)	105-108
Number of pages	4
ISSN	1438-5163
DOIs	https://doi.org/10.1002/1438-5171(200107)2:2<105::AID-SIMO105>3.0.CO;2-0
Publication status	Published - 1 Jan 2001

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10.1002/1438-5171(200107)2:2<105::AID-SIMO105>3.0.CO;2-0

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title = "In situ atomic force microscope imaging of supported lipid bilayers",

abstract = "In situ AFM images of phospholipase A2 (PLA2) hydrolysis of mica-supported one- and two-component lipid bilayers are presented. For one-component DPPC bilayers an enhanced enzymatic activity is observed towards preexisting defects in the bilayer. Phase separation is observed in two-component DMPC-DSPC bilayers and a remarkable enhanced hydrolytic activity of the PLA2-enzyme for the DMPC-rich phase is seen. Furthermore, in a supported double bilayer system a characteristic ripple structure, most likely related to the formation of the Pβ'-ripple phase is observed.",

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T1 - In situ atomic force microscope imaging of supported lipid bilayers

AU - Kaasgaard, Thomas

AU - Leidy, Chad

AU - Ipsen, John Hjort

AU - Mouritsen, Ole G.

AU - Jørgensen, Kent

PY - 2001/1/1

Y1 - 2001/1/1

N2 - In situ AFM images of phospholipase A2 (PLA2) hydrolysis of mica-supported one- and two-component lipid bilayers are presented. For one-component DPPC bilayers an enhanced enzymatic activity is observed towards preexisting defects in the bilayer. Phase separation is observed in two-component DMPC-DSPC bilayers and a remarkable enhanced hydrolytic activity of the PLA2-enzyme for the DMPC-rich phase is seen. Furthermore, in a supported double bilayer system a characteristic ripple structure, most likely related to the formation of the Pβ'-ripple phase is observed.

AB - In situ AFM images of phospholipase A2 (PLA2) hydrolysis of mica-supported one- and two-component lipid bilayers are presented. For one-component DPPC bilayers an enhanced enzymatic activity is observed towards preexisting defects in the bilayer. Phase separation is observed in two-component DMPC-DSPC bilayers and a remarkable enhanced hydrolytic activity of the PLA2-enzyme for the DMPC-rich phase is seen. Furthermore, in a supported double bilayer system a characteristic ripple structure, most likely related to the formation of the Pβ'-ripple phase is observed.

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SP - 105

EP - 108

JO - Single Molecules

JF - Single Molecules

SN - 1438-5163

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In situ atomic force microscope imaging of supported lipid bilayers

Abstract

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