In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts

Jean Y Perentes; Trevor D McKee; Carsten D Ley; Hannah Mathiew; Michelle Dawson; Timothy P Padera; Lance L Munn; Rakesh K Jain; Yves Boucher

doi:10.1038/nmeth.1295

In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts

Jean Y Perentes, Trevor D McKee, Carsten D Ley, Hannah Mathiew, Michelle Dawson, Timothy P Padera, Lance L Munn, Rakesh K Jain, Yves Boucher

Teaching

Research output: Contribution to journal › Journal article › peer-review

109 Citations (Scopus)

Abstract

Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.

Original language	English
Journal	Nature Methods
Volume	6
Issue number	2
Pages (from-to)	143-5
Number of pages	2
ISSN	1548-7091
DOIs	https://doi.org/10.1038/nmeth.1295
Publication status	Published - 2009

Bibliographical note

Keywords: Animals; Cell Line, Tumor; Cell Movement; Collagen; Extracellular Matrix; Fibroblasts; Humans; Image Enhancement; Integrin beta Chains; Mice; Mice, Transgenic; Microscopy, Confocal; Relaxin; Soft Tissue Neoplasms; Stromal Cells

Access to Document

10.1038/nmeth.1295

Cite this

@article{9803eaf0359f11df8ed1000ea68e967b,

title = "In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts",

abstract = "Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.",

author = "Perentes, {Jean Y} and McKee, {Trevor D} and Ley, {Carsten D} and Hannah Mathiew and Michelle Dawson and Padera, {Timothy P} and Munn, {Lance L} and Jain, {Rakesh K} and Yves Boucher",

note = "Keywords: Animals; Cell Line, Tumor; Cell Movement; Collagen; Extracellular Matrix; Fibroblasts; Humans; Image Enhancement; Integrin beta Chains; Mice; Mice, Transgenic; Microscopy, Confocal; Relaxin; Soft Tissue Neoplasms; Stromal Cells",

year = "2009",

doi = "10.1038/nmeth.1295",

language = "English",

volume = "6",

pages = "143--5",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "nature publishing group",

number = "2",

}

TY - JOUR

T1 - In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts

AU - Perentes, Jean Y

AU - McKee, Trevor D

AU - Ley, Carsten D

AU - Mathiew, Hannah

AU - Dawson, Michelle

AU - Padera, Timothy P

AU - Munn, Lance L

AU - Jain, Rakesh K

AU - Boucher, Yves

N1 - Keywords: Animals; Cell Line, Tumor; Cell Movement; Collagen; Extracellular Matrix; Fibroblasts; Humans; Image Enhancement; Integrin beta Chains; Mice; Mice, Transgenic; Microscopy, Confocal; Relaxin; Soft Tissue Neoplasms; Stromal Cells

PY - 2009

Y1 - 2009

N2 - Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.

AB - Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.

U2 - 10.1038/nmeth.1295

DO - 10.1038/nmeth.1295

M3 - Journal article

C2 - 19151720

VL - 6

SP - 143

EP - 145

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 2

ER -