Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears

Robbert J. Van der Pijl; Weikang Ma; Christopher T.A. Lewis; Line Haar; Amalie Buhl; Gerrie P. Farman; Marcus Rhodehamel; Vivek P. Jani; O. Lynne Nelson; Chengxin Zhang; Henk Granzier; Julien Ochala

doi:10.1016/j.molmet.2024.102084

Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears

Robbert J. Van der Pijl, Weikang Ma, Christopher T.A. Lewis, Line Haar, Amalie Buhl, Gerrie P. Farman, Marcus Rhodehamel, Vivek P. Jani, O. Lynne Nelson, Chengxin Zhang, Henk Granzier, Julien Ochala^*

^*Corresponding author for this work

Exercise and Muscle

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Aim: The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals. Methods: Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics. Main findings: Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability. Conclusions: Cardiac myosin depresses the heart's energetic demand during hibernation by modulating its function.

Original language	English
Article number	102084
Journal	Molecular Metabolism
Volume	92
Number of pages	8
ISSN	2212-8778
DOIs	https://doi.org/10.1016/j.molmet.2024.102084
Publication status	Published - 2025

Bibliographical note

Publisher Copyright:
© 2024 The Author(s)

Keywords

Heart
Hibernation
Metabolism
Myosin

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Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears. / Van der Pijl, Robbert J.; Ma, Weikang; Lewis, Christopher T.A.; Haar, Line; Buhl, Amalie; Farman, Gerrie P.; Rhodehamel, Marcus; Jani, Vivek P.; Nelson, O. Lynne; Zhang, Chengxin; Granzier, Henk; Ochala, Julien.

In: Molecular Metabolism, Vol. 92, 102084, 2025.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "Aim: The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals. Methods: Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics. Main findings: Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability. Conclusions: Cardiac myosin depresses the heart's energetic demand during hibernation by modulating its function.",

keywords = "Heart, Hibernation, Metabolism, Myosin",

author = "{Van der Pijl}, {Robbert J.} and Weikang Ma and Lewis, {Christopher T.A.} and Line Haar and Amalie Buhl and Farman, {Gerrie P.} and Marcus Rhodehamel and Jani, {Vivek P.} and Nelson, {O. Lynne} and Chengxin Zhang and Henk Granzier and Julien Ochala",

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doi = "10.1016/j.molmet.2024.102084",

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T1 - Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears

AU - Van der Pijl, Robbert J.

AU - Ma, Weikang

AU - Lewis, Christopher T.A.

AU - Haar, Line

AU - Buhl, Amalie

AU - Farman, Gerrie P.

AU - Rhodehamel, Marcus

AU - Jani, Vivek P.

AU - Nelson, O. Lynne

AU - Zhang, Chengxin

AU - Granzier, Henk

AU - Ochala, Julien

PY - 2025

Y1 - 2025

N2 - Aim: The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals. Methods: Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics. Main findings: Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability. Conclusions: Cardiac myosin depresses the heart's energetic demand during hibernation by modulating its function.

AB - Aim: The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals. Methods: Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics. Main findings: Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability. Conclusions: Cardiac myosin depresses the heart's energetic demand during hibernation by modulating its function.

KW - Heart

KW - Hibernation

KW - Metabolism

KW - Myosin

U2 - 10.1016/j.molmet.2024.102084

DO - 10.1016/j.molmet.2024.102084

M3 - Journal article

C2 - 39694092

AN - SCOPUS:85212860782

VL - 92

JO - Molecular Metabolism

JF - Molecular Metabolism

SN - 2212-8778

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