Indirect immunofluorescence staining and crossed immunoelectrophoresis for differentiation of Candida albicans and Geotrichum candidum

H. E. Jensen*, J. Hau, B. Aalbaek, H. Schonheyder

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

17 Citations (Scopus)

Abstract

Rabbit hyperimmune antisera were used for differential diagnosis of Candida albicans and Geotrichum candidum in murine and bovine tissues by indirect immunofluorescence (IIF) staining. Although the antisera did not detect common antigens by crossed immunoelectrophoresis (XIE) a marked cross-reactivity of fungal cells was observed in the IIF assay. Following liquid phase absorption with heterologous antigenic preparations the hyperimmune antisera were rendered specific for each species thus making possible an unequivocal differentiation of the two species.

Original languageEnglish
Book seriesMycoses
Volume33
Issue number11-12
Pages (from-to)519-526
Number of pages8
ISSN0933-7407
DOIs
Publication statusPublished - 1990

Keywords

  • Candida albicans
  • crossed immunoelectrophoresis
  • Geotrichum candidum
  • immunofluorescence staining
  • immunological differentiation

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