Induction of DNA damage by oxidised amino acids and proteins

Catherine Luxford, Roger T Dean, Michael Jonathan Davies

Research output: Contribution to journalJournal articleResearchpeer-review

45 Citations (Scopus)

Abstract

Exposure of amino acids, peptides and proteins to radicals in the presence of O2 generates hydroperoxides in a dose-dependent manner. These hydroperoxides are stable in the absence of exogenous catalysts (e.g. heat, light, redox-active transition metal ions), but decompose rapidly in the presence of these agents to give a variety of radicals including alkoxyl (RO*), peroxyl (ROO*) and carbon-centred (R) species. These radicals are shown to react with DNA to give DNA-protein cross-links and single strand breaks.

Original languageEnglish
JournalBiogerontology
Volume3
Issue number1-2
Pages (from-to)95-102
Number of pages8
ISSN1389-5729
Publication statusPublished - 2002

Keywords

  • Amino Acids
  • Animals
  • Cattle
  • DNA Damage
  • Electrophoresis, Agar Gel
  • Hydrogen Peroxide
  • Oxidation-Reduction
  • Proteins

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