Influence of the OGG1 Ser326Cys polymorphism on oxidatively damaged DNA and repair activity

Annie Jensen; Mille Løhr; Louise Eriksen; Morten Grønbæk; Elad Dorry; Steffen Loft; Peter Møller

doi:10.1016/j.freeradbiomed.2011.09.038

Influence of the OGG1 Ser326Cys polymorphism on oxidatively damaged DNA and repair activity

Annie Jensen, Mille Løhr, Louise Eriksen, Morten Grønbæk, Elad Dorry, Steffen Loft, Peter Møller

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Oxidatively damaged DNA base lesions are considered to be mainly repaired by 8-oxoguanine DNA glycosylase (OGG1) mediated pathways. We investigated the effect of the OGG1 Ser326Cys polymorphism on the level and repair of oxidatively damaged DNA in mononuclear blood cells (MNBC) by means of the comet assay. We collected blood samples from 1,019 healthy subjects and genotyped for the OGG1 Ser326Cys polymorphism. We found 49 subjects homozygous for the variant genotype (Cys/Cys) and selected same numbers of age-matched subjects with the heterozygous (Ser/Cys) and homozygous wild-type genotype (Ser/Ser). Carriers of the Cys/Cys genotype had higher levels of formamidopyrimidine DNA glycosylase (FPG) sensitive sites in MNBC (0.31 ± 0.03 lesions/10(6)bp) compared to Ser/Ser (0.19 ± 0.02 lesions/10(6)bp, P

Original language	English
Journal	Free Radical Biology & Medicine
Volume	52
Issue number	1
Pages (from-to)	118-25
Number of pages	8
ISSN	0891-5849
DOIs	https://doi.org/10.1016/j.freeradbiomed.2011.09.038
Publication status	Published - 2012

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10.1016/j.freeradbiomed.2011.09.038

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@article{708942f2e0de42688c96a3f110b89adf,

title = "Influence of the OGG1 Ser326Cys polymorphism on oxidatively damaged DNA and repair activity",

abstract = "Oxidatively damaged DNA base lesions are considered to be mainly repaired by 8-oxoguanine DNA glycosylase (OGG1) mediated pathways. We investigated the effect of the OGG1 Ser326Cys polymorphism on the level and repair of oxidatively damaged DNA in mononuclear blood cells (MNBC) by means of the comet assay. We collected blood samples from 1,019 healthy subjects and genotyped for the OGG1 Ser326Cys polymorphism. We found 49 subjects homozygous for the variant genotype (Cys/Cys) and selected same numbers of age-matched subjects with the heterozygous (Ser/Cys) and homozygous wild-type genotype (Ser/Ser). Carriers of the Cys/Cys genotype had higher levels of formamidopyrimidine DNA glycosylase (FPG) sensitive sites in MNBC (0.31 ± 0.03 lesions/10(6)bp) compared to Ser/Ser (0.19 ± 0.02 lesions/10(6)bp, P",

author = "Annie Jensen and Mille L{\o}hr and Louise Eriksen and Morten Gr{\o}nb{\ae}k and Elad Dorry and Steffen Loft and Peter M{\o}ller",

year = "2012",

doi = "10.1016/j.freeradbiomed.2011.09.038",

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volume = "52",

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T1 - Influence of the OGG1 Ser326Cys polymorphism on oxidatively damaged DNA and repair activity

AU - Jensen, Annie

AU - Løhr, Mille

AU - Eriksen, Louise

AU - Grønbæk, Morten

AU - Dorry, Elad

AU - Loft, Steffen

AU - Møller, Peter

PY - 2012

Y1 - 2012

N2 - Oxidatively damaged DNA base lesions are considered to be mainly repaired by 8-oxoguanine DNA glycosylase (OGG1) mediated pathways. We investigated the effect of the OGG1 Ser326Cys polymorphism on the level and repair of oxidatively damaged DNA in mononuclear blood cells (MNBC) by means of the comet assay. We collected blood samples from 1,019 healthy subjects and genotyped for the OGG1 Ser326Cys polymorphism. We found 49 subjects homozygous for the variant genotype (Cys/Cys) and selected same numbers of age-matched subjects with the heterozygous (Ser/Cys) and homozygous wild-type genotype (Ser/Ser). Carriers of the Cys/Cys genotype had higher levels of formamidopyrimidine DNA glycosylase (FPG) sensitive sites in MNBC (0.31 ± 0.03 lesions/10(6)bp) compared to Ser/Ser (0.19 ± 0.02 lesions/10(6)bp, P

AB - Oxidatively damaged DNA base lesions are considered to be mainly repaired by 8-oxoguanine DNA glycosylase (OGG1) mediated pathways. We investigated the effect of the OGG1 Ser326Cys polymorphism on the level and repair of oxidatively damaged DNA in mononuclear blood cells (MNBC) by means of the comet assay. We collected blood samples from 1,019 healthy subjects and genotyped for the OGG1 Ser326Cys polymorphism. We found 49 subjects homozygous for the variant genotype (Cys/Cys) and selected same numbers of age-matched subjects with the heterozygous (Ser/Cys) and homozygous wild-type genotype (Ser/Ser). Carriers of the Cys/Cys genotype had higher levels of formamidopyrimidine DNA glycosylase (FPG) sensitive sites in MNBC (0.31 ± 0.03 lesions/10(6)bp) compared to Ser/Ser (0.19 ± 0.02 lesions/10(6)bp, P

U2 - 10.1016/j.freeradbiomed.2011.09.038

DO - 10.1016/j.freeradbiomed.2011.09.038

M3 - Journal article

C2 - 22019439

VL - 52

SP - 118

EP - 125

JO - Free Radical Biology & Medicine

JF - Free Radical Biology & Medicine

SN - 0891-5849

IS - 1

ER -