Insulin secretion and cellular glucose metabolism after prolonged low-grade intralipid infusion in young men

Christine B Jensen, Heidi Storgaard, Jens Juul Holst, Flemming Dela, Sten Madsbad, Allan A Vaag

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Abstract

We examined the simultaneous effects of a 24-h low-grade Intralipid infusion on peripheral glucose disposal, intracellular glucose partitioning and insulin secretion rates in twenty young men, by 2-step hyperinsulinemic euglycemic clamp [low insulin clamp (LI), 10 mU/m(2) x min; high insulin clamp (HI), 40 mU/m(2) x min], 3-(3)H-glucose, indirect calorimetry, and iv glucose tolerance test. Free fatty acid concentrations were similar during basal steady state but 3.7- to 13-fold higher during clamps. P-glucagon increased and the insulin/glucagon ratio decreased at both LI and HI during Intralipid infusion. At LI, glucose oxidation decreased by 10%, whereas glucose disposal, glycolytic flux, glucose storage, and glucose production were not significantly altered. At HI, glucose disposal, and glucose oxidation decreased by 12% and 24%, respectively, during Intralipid infusion. Glycolytic flux, glucose storage, and glucose production were unchanged. Insulin secretion rates increased in response to Intralipid infusion, but disposition indices (DI = insulin action.insulin secretion) were unchanged. In conclusion, a 24-h low-grade Intralipid infusion caused insulin resistance in the oxidative (but not in the nonoxidative) glucose metabolism in young healthy men. Moreover, insulin hypersecretion perfectly countered the free-fatty acid-induced insulin resistance. Future studies are needed to determine the role of a prolonged moderate lipid load in subjects at increased risk of developing diabetes.

Original languageEnglish
JournalThe Journal of clinical endocrinology and metabolism
Volume88
Issue number6
Pages (from-to)2775-83
Number of pages9
ISSN0021-972X
DOIs
Publication statusPublished - Jun 2003

Keywords

  • Adult
  • Area Under Curve
  • Blood Glucose
  • C-Peptide
  • Fat Emulsions, Intravenous
  • Fatty Acids, Nonesterified
  • Glucagon
  • Glucose
  • Glucose Tolerance Test
  • Humans
  • Insulin
  • Insulin Resistance
  • Male
  • Oxidation-Reduction

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