Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydrooxoguanine/8-oxo-7,8-dihydro-2`-deoxyguanosine in DNA

A.R. Collins; C.M. Gedik; S. Wood; A. White; J. Bubois; P. Duez; J.F. Rees; R. Legall; L. Degand; Steffen Loft; A. Jensen; H. Poulsen; A. Weimann; B.R. Jensen; J. Cadet; Thierry Douki; J.L. Ravanat; H. Faure; M. Tripier; I. Morel; O. Sergent; P. Cillard; B. Morin; B. Epe; N. Phoa; A. Hartwig; A. Pelzer; P. Dolara; C. Casalini; F. Guglielmi; C. Luceri; H. Kasai; R. Kido; R. Olinski; K. Bialkowski; Z. Duracková; L. Hlinchikova; P. Korytar; M. Dusinska; C. Mislanova; J. Vina; A. Lloret; L. Möller; T. Hofer; E. Gremaud; L. Fay; R. Stadler; J. Eakins; F. Progna; J. O`Brien; R. Elliott; S.B. Astley; A. Bailley; K. Herbert; D. Chauhan; F. Kelly; C. Dunster; J. Lunec; I. Podmore; P. Patel; S. Johnson; M. Evans; A. White; R. Tyrrell; Matthew Gordon; Chris Wild; Laura Hardie

doi:10.1080/10715760290019246

Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydrooxoguanine/8-oxo-7,8-dihydro-2`-deoxyguanosine in DNA

A.R. Collins, C.M. Gedik, S. Wood, A. White, J. Bubois, P. Duez, J.F. Rees, R. Legall, L. Degand, Steffen Loft, A. Jensen, H. Poulsen, A. Weimann, B.R. Jensen, J. Cadet, Thierry Douki, J.L. Ravanat, H. Faure, M. Tripier, I. MorelO. Sergent, P. Cillard, B. Morin, B. Epe, N. Phoa, A. Hartwig, A. Pelzer, P. Dolara, C. Casalini, F. Guglielmi, C. Luceri, H. Kasai, R. Kido, R. Olinski, K. Bialkowski, Z. Duracková, L. Hlinchikova, P. Korytar, M. Dusinska, C. Mislanova, J. Vina, A. Lloret, L. Möller, T. Hofer, E. Gremaud, L. Fay, R. Stadler, J. Eakins, F. Progna, J. O`Brien, R. Elliott, S.B. Astley, A. Bailley, K. Herbert, D. Chauhan, F. Kelly, C. Dunster, J. Lunec, I. Podmore, P. Patel, S. Johnson, M. Evans, A. White, R. Tyrrell, Matthew Gordon, Chris Wild, Laura Hardie

Research output: Contribution to journal › Journal article › Research › peer-review

78 Citations (Scopus)

Abstract

The aim of ESCODD, a European Commission funded Concerted Action, is to improve the precision and accuracy of methods for measuring 8-oxo-7,8-dihydroguanine (8-oxoGua) or the nucleoside (8-oxodG). On two occasions, participating laboratories received samples of different concentrations of 8-oxodG for analysis. About half the results returned (for 8-oxodG) were within 20% of the median values. Coefficients of variation (for three identical samples) were commonly around 10%. A sample of calf thymus DNA was sent, dry, to all laboratories. Analysis of 8-oxoGua/8-oxodG in this sample was a test of hydrolysis methods. Almost half the reported results were within 20% of the median value, and half obtained a CVof less than 10%. In order to test sensitivity, as well as precision, DNA was treated with photosensitiser and light to introduce increasing amounts of 8-oxoGua and samples were sent to members. Median values calculated from all returned results were 45.6 (untreated), 53.9, 60.4 and 65.6 8-oxoGua/10(6) Gua; only seven laboratories detected the increase over the whole range, while all but one detected a dose response over two concentration intervals. Results in this trial reflect a continuing improvement in precision and accuracy. The next challenge will be the analysis of 8-oxodG in DNA isolated from cells or tissue, where the concentration is much lower than in calf thymus DNA.

Original language	English
Journal	Free Radical Research
Issue number	vol. 36
Pages (from-to)	239-245
ISSN	1071-5762
DOIs	https://doi.org/10.1080/10715760290019246
Publication status	Published - 2002

Access to Document

10.1080/10715760290019246

Library access?

Check availability at the Royal Danish Library

Cite this

Collins, A. R., Gedik, C. M., Wood, S., White, A., Bubois, J., Duez, P., Rees, J. F., Legall, R., Degand, L., Loft, S., Jensen, A., Poulsen, H., Weimann, A., Jensen, B. R., Cadet, J., Douki, T., Ravanat, J. L., Faure, H., Tripier, M., ... Hardie, L. (2002). Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydrooxoguanine/8-oxo-7,8-dihydro-2`-deoxyguanosine in DNA. Free Radical Research, (vol. 36), 239-245. https://doi.org/10.1080/10715760290019246

Collins, AR, Gedik, CM, Wood, S, White, A, Bubois, J, Duez, P, Rees, JF, Legall, R, Degand, L, Loft, S, Jensen, A, Poulsen, H, Weimann, A, Jensen, BR, Cadet, J, Douki, T, Ravanat, JL, Faure, H, Tripier, M, Morel, I, Sergent, O, Cillard, P, Morin, B, Epe, B, Phoa, N, Hartwig, A, Pelzer, A, Dolara, P, Casalini, C, Guglielmi, F, Luceri, C, Kasai, H, Kido, R, Olinski, R, Bialkowski, K, Duracková, Z, Hlinchikova, L, Korytar, P, Dusinska, M, Mislanova, C, Vina, J, Lloret, A, Möller, L, Hofer, T, Gremaud, E, Fay, L, Stadler, R, Eakins, J, Progna, F, O`Brien, J, Elliott, R, Astley, SB, Bailley, A, Herbert, K, Chauhan, D, Kelly, F, Dunster, C, Lunec, J, Podmore, I, Patel, P, Johnson, S, Evans, M, White, A, Tyrrell, R, Gordon, M, Wild, C & Hardie, L 2002, 'Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydrooxoguanine/8-oxo-7,8-dihydro-2`-deoxyguanosine in DNA', Free Radical Research, no. vol. 36, pp. 239-245. https://doi.org/10.1080/10715760290019246

@article{d49a416074c511dbbee902004c4f4f50,

title = "Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydrooxoguanine/8-oxo-7,8-dihydro-2`-deoxyguanosine in DNA",

abstract = "The aim of ESCODD, a European Commission funded Concerted Action, is to improve the precision and accuracy of methods for measuring 8-oxo-7,8-dihydroguanine (8-oxoGua) or the nucleoside (8-oxodG). On two occasions, participating laboratories received samples of different concentrations of 8-oxodG for analysis. About half the results returned (for 8-oxodG) were within 20% of the median values. Coefficients of variation (for three identical samples) were commonly around 10%. A sample of calf thymus DNA was sent, dry, to all laboratories. Analysis of 8-oxoGua/8-oxodG in this sample was a test of hydrolysis methods. Almost half the reported results were within 20% of the median value, and half obtained a CVof less than 10%. In order to test sensitivity, as well as precision, DNA was treated with photosensitiser and light to introduce increasing amounts of 8-oxoGua and samples were sent to members. Median values calculated from all returned results were 45.6 (untreated), 53.9, 60.4 and 65.6 8-oxoGua/10(6) Gua; only seven laboratories detected the increase over the whole range, while all but one detected a dose response over two concentration intervals. Results in this trial reflect a continuing improvement in precision and accuracy. The next challenge will be the analysis of 8-oxodG in DNA isolated from cells or tissue, where the concentration is much lower than in calf thymus DNA.",

author = "A.R. Collins and C.M. Gedik and S. Wood and A. White and J. Bubois and P. Duez and J.F. Rees and R. Legall and L. Degand and Steffen Loft and A. Jensen and H. Poulsen and A. Weimann and B.R. Jensen and J. Cadet and Thierry Douki and J.L. Ravanat and H. Faure and M. Tripier and I. Morel and O. Sergent and P. Cillard and B. Morin and B. Epe and N. Phoa and A. Hartwig and A. Pelzer and P. Dolara and C. Casalini and F. Guglielmi and C. Luceri and H. Kasai and R. Kido and R. Olinski and K. Bialkowski and Z. Durackov{\'a} and L. Hlinchikova and P. Korytar and M. Dusinska and C. Mislanova and J. Vina and A. Lloret and L. M{\"o}ller and T. Hofer and E. Gremaud and L. Fay and R. Stadler and J. Eakins and F. Progna and J. O`Brien and R. Elliott and S.B. Astley and A. Bailley and K. Herbert and D. Chauhan and F. Kelly and C. Dunster and J. Lunec and I. Podmore and P. Patel and S. Johnson and M. Evans and A. White and R. Tyrrell and Matthew Gordon and Chris Wild and Laura Hardie",

note = "Paper id:: 12071341",

year = "2002",

doi = "10.1080/10715760290019246",

language = "English",

pages = "239--245",

journal = "Free Radical Research",

issn = "1071-5762",

publisher = "Taylor & Francis",

number = "vol. 36",

}

TY - JOUR

T1 - Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydrooxoguanine/8-oxo-7,8-dihydro-2`-deoxyguanosine in DNA

AU - Collins, A.R.

AU - Gedik, C.M.

AU - Wood, S.

AU - White, A.

AU - Bubois, J.

AU - Duez, P.

AU - Rees, J.F.

AU - Legall, R.

AU - Degand, L.

AU - Loft, Steffen

AU - Jensen, A.

AU - Poulsen, H.

AU - Weimann, A.

AU - Jensen, B.R.

AU - Cadet, J.

AU - Douki, Thierry

AU - Ravanat, J.L.

AU - Faure, H.

AU - Tripier, M.

AU - Morel, I.

AU - Sergent, O.

AU - Cillard, P.

AU - Morin, B.

AU - Epe, B.

AU - Phoa, N.

AU - Hartwig, A.

AU - Pelzer, A.

AU - Dolara, P.

AU - Casalini, C.

AU - Guglielmi, F.

AU - Luceri, C.

AU - Kasai, H.

AU - Kido, R.

AU - Olinski, R.

AU - Bialkowski, K.

AU - Duracková, Z.

AU - Hlinchikova, L.

AU - Korytar, P.

AU - Dusinska, M.

AU - Mislanova, C.

AU - Vina, J.

AU - Lloret, A.

AU - Möller, L.

AU - Hofer, T.

AU - Gremaud, E.

AU - Fay, L.

AU - Stadler, R.

AU - Eakins, J.

AU - Progna, F.

AU - O`Brien, J.

AU - Elliott, R.

AU - Astley, S.B.

AU - Bailley, A.

AU - Herbert, K.

AU - Chauhan, D.

AU - Kelly, F.

AU - Dunster, C.

AU - Lunec, J.

AU - Podmore, I.

AU - Patel, P.

AU - Johnson, S.

AU - Evans, M.

AU - White, A.

AU - Tyrrell, R.

AU - Gordon, Matthew

AU - Wild, Chris

AU - Hardie, Laura

N1 - Paper id:: 12071341

PY - 2002

Y1 - 2002

N2 - The aim of ESCODD, a European Commission funded Concerted Action, is to improve the precision and accuracy of methods for measuring 8-oxo-7,8-dihydroguanine (8-oxoGua) or the nucleoside (8-oxodG). On two occasions, participating laboratories received samples of different concentrations of 8-oxodG for analysis. About half the results returned (for 8-oxodG) were within 20% of the median values. Coefficients of variation (for three identical samples) were commonly around 10%. A sample of calf thymus DNA was sent, dry, to all laboratories. Analysis of 8-oxoGua/8-oxodG in this sample was a test of hydrolysis methods. Almost half the reported results were within 20% of the median value, and half obtained a CVof less than 10%. In order to test sensitivity, as well as precision, DNA was treated with photosensitiser and light to introduce increasing amounts of 8-oxoGua and samples were sent to members. Median values calculated from all returned results were 45.6 (untreated), 53.9, 60.4 and 65.6 8-oxoGua/10(6) Gua; only seven laboratories detected the increase over the whole range, while all but one detected a dose response over two concentration intervals. Results in this trial reflect a continuing improvement in precision and accuracy. The next challenge will be the analysis of 8-oxodG in DNA isolated from cells or tissue, where the concentration is much lower than in calf thymus DNA.

AB - The aim of ESCODD, a European Commission funded Concerted Action, is to improve the precision and accuracy of methods for measuring 8-oxo-7,8-dihydroguanine (8-oxoGua) or the nucleoside (8-oxodG). On two occasions, participating laboratories received samples of different concentrations of 8-oxodG for analysis. About half the results returned (for 8-oxodG) were within 20% of the median values. Coefficients of variation (for three identical samples) were commonly around 10%. A sample of calf thymus DNA was sent, dry, to all laboratories. Analysis of 8-oxoGua/8-oxodG in this sample was a test of hydrolysis methods. Almost half the reported results were within 20% of the median value, and half obtained a CVof less than 10%. In order to test sensitivity, as well as precision, DNA was treated with photosensitiser and light to introduce increasing amounts of 8-oxoGua and samples were sent to members. Median values calculated from all returned results were 45.6 (untreated), 53.9, 60.4 and 65.6 8-oxoGua/10(6) Gua; only seven laboratories detected the increase over the whole range, while all but one detected a dose response over two concentration intervals. Results in this trial reflect a continuing improvement in precision and accuracy. The next challenge will be the analysis of 8-oxodG in DNA isolated from cells or tissue, where the concentration is much lower than in calf thymus DNA.

U2 - 10.1080/10715760290019246

DO - 10.1080/10715760290019246

M3 - Journal article

SN - 1071-5762

SP - 239

EP - 245

JO - Free Radical Research

JF - Free Radical Research

IS - vol. 36

ER -