TY - JOUR
T1 - Inter-vendor variance of enteric eukaryotic DNA viruses in specific pathogen free C57BL/6N mice
AU - Rasmussen, Torben Sølbeck
AU - Jakobsen, Rasmus Riemer
AU - Castro-Mejía, Josué L.
AU - Kot, Witold
AU - Thomsen, Allan Randrup
AU - Vogensen, Finn Kvist
AU - Nielsen, Dennis Sandris
AU - Hansen, Axel Kornerup
PY - 2021
Y1 - 2021
N2 - The laboratory mouse strain C57BL/6 is widely used as an animal model for various applications. It is becoming increasingly clear that the bacterial enteric community highly influences the phenotype. Eukaryotic viruses represent a sparsely investigated member of the enteric microbiome that might also affect the phenotype. We here investigated the presence of enteric eukaryotic DNA viruses (EDVs) in specific pathogen-free (SPF) C57BL/6N mice purchased from three vendors upon arrival and after being fed a low-fat diet (LFD) or high-fat diet (HFD). We detected genetic fragments of EDVs belonging to the viral families of Herpes-, Mimi-, Baculo- and Phycodnaviridae represented by two genera; Chlorovirus and Prasinovirus. The EDVs were detected in the mice upon arrival and persisted for 13 weeks. However, these signals of EDVs were only detected at notable levels in mice fed LFD from 2 out of 3 vendors, which suggested that the enteric composition of these EDVs were affected by both vendor (p < 0.003) and different dietary regimes (p < 0.013). This highlights the need of additional studies assessing the potential function of these EDVs that may influence the mouse phenotype and the reproducibility of animal studies using this C57BL/6N substrain.
AB - The laboratory mouse strain C57BL/6 is widely used as an animal model for various applications. It is becoming increasingly clear that the bacterial enteric community highly influences the phenotype. Eukaryotic viruses represent a sparsely investigated member of the enteric microbiome that might also affect the phenotype. We here investigated the presence of enteric eukaryotic DNA viruses (EDVs) in specific pathogen-free (SPF) C57BL/6N mice purchased from three vendors upon arrival and after being fed a low-fat diet (LFD) or high-fat diet (HFD). We detected genetic fragments of EDVs belonging to the viral families of Herpes-, Mimi-, Baculo- and Phycodnaviridae represented by two genera; Chlorovirus and Prasinovirus. The EDVs were detected in the mice upon arrival and persisted for 13 weeks. However, these signals of EDVs were only detected at notable levels in mice fed LFD from 2 out of 3 vendors, which suggested that the enteric composition of these EDVs were affected by both vendor (p < 0.003) and different dietary regimes (p < 0.013). This highlights the need of additional studies assessing the potential function of these EDVs that may influence the mouse phenotype and the reproducibility of animal studies using this C57BL/6N substrain.
KW - Animal model reproducibility
KW - Chlorovirus
KW - Eukaryotic DNA viruses
KW - Gut microbiome
KW - Specific pathogen free mice
KW - Vendor effect
KW - Virome
U2 - 10.1016/j.rvsc.2021.01.022
DO - 10.1016/j.rvsc.2021.01.022
M3 - Journal article
C2 - 33548686
AN - SCOPUS:85100397702
VL - 136
SP - 1
EP - 5
JO - Research in Veterinary Science
JF - Research in Veterinary Science
SN - 0034-5288
ER -