TY - JOUR
T1 - Interdisciplinary Synergy to Reveal Mechanisms of Annexin-Mediated Plasma Membrane Shaping and Repair
AU - Bendix, Poul Martin
AU - Simonsen, Adam Cohen
AU - Florentsen, Christoffer D
AU - Häger, Swantje Christin
AU - Mularski, Anna
AU - Zanjani, Ali Asghar Hakami
AU - Moreno-Pescador, Guillermo
AU - Klenow, Martin Berg
AU - Sønder, Stine Lauritzen
AU - Danielsen, Helena M
AU - Arastoo, Mohammad Reza
AU - Heitmann, Anne Sofie
AU - Pandey, Mayank Prakash
AU - Lund, Frederik Wendelboe
AU - Dias, Catarina
AU - Khandelia, Himanshu
AU - Nylandsted, Jesper
PY - 2020/4/21
Y1 - 2020/4/21
N2 - The plasma membrane surrounds every single cell and essentially shapes cell life by separating the interior from the external environment. Thus, maintenance of cell membrane integrity is essential to prevent death caused by disruption of the plasma membrane. To counteract plasma membrane injuries, eukaryotic cells have developed efficient repair tools that depend on Ca2+- and phospholipid-binding annexin proteins. Upon membrane damage, annexin family members are activated by a Ca2+ influx, enabling them to quickly bind at the damaged membrane and facilitate wound healing. Our recent studies, based on interdisciplinary research synergy across molecular cell biology, experimental membrane physics, and computational simulations show that annexins have additional biophysical functions in the repair response besides enabling membrane fusion. Annexins possess different membrane-shaping properties, allowing for a tailored response that involves rapid bending, constriction, and fusion of membrane edges for resealing. Moreover, some annexins have high affinity for highly curved membranes that appear at free edges near rupture sites, a property that might accelerate their recruitment for rapid repair. Here, we discuss the mechanisms of annexin-mediated membrane shaping and curvature sensing in the light of our interdisciplinary approach to study plasma membrane repair.
AB - The plasma membrane surrounds every single cell and essentially shapes cell life by separating the interior from the external environment. Thus, maintenance of cell membrane integrity is essential to prevent death caused by disruption of the plasma membrane. To counteract plasma membrane injuries, eukaryotic cells have developed efficient repair tools that depend on Ca2+- and phospholipid-binding annexin proteins. Upon membrane damage, annexin family members are activated by a Ca2+ influx, enabling them to quickly bind at the damaged membrane and facilitate wound healing. Our recent studies, based on interdisciplinary research synergy across molecular cell biology, experimental membrane physics, and computational simulations show that annexins have additional biophysical functions in the repair response besides enabling membrane fusion. Annexins possess different membrane-shaping properties, allowing for a tailored response that involves rapid bending, constriction, and fusion of membrane edges for resealing. Moreover, some annexins have high affinity for highly curved membranes that appear at free edges near rupture sites, a property that might accelerate their recruitment for rapid repair. Here, we discuss the mechanisms of annexin-mediated membrane shaping and curvature sensing in the light of our interdisciplinary approach to study plasma membrane repair.
U2 - 10.3390/cells9041029
DO - 10.3390/cells9041029
M3 - Review
C2 - 32326222
VL - 9
JO - Cells
JF - Cells
SN - 2073-4409
IS - 4
M1 - 1029
ER -