Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes

Hélène Bouvrais; Flemming Cornelius; John H. Ipsen; Ole G. Mouritsen

doi:10.1073/pnas.1209909109

Intrinsic reaction-cycle time scale of Na⁺,K⁺-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes

Hélène Bouvrais, Flemming Cornelius, John H. Ipsen, Ole G. Mouritsen

Research output: Contribution to journal › Journal article › Research › peer-review

36 Citations (Scopus)

Abstract

Interaction between integral membrane proteins and the lipid-bilayer component of biological membranes is expected to mutually infl uence the proteins and the membrane. We present quantitative evidence of a manifestation of the lipid-protein interactions in liposomal membranes, reconstituted with actively pumping Na⁺,K⁺-ATPase, in terms of nonequilibrium shape fluctuations that contain a relaxation time, τ, which is robust and independent of the speci fi c fl uctuation modes of the membrane. In the case of pumping Na⁺-ions, analysis of the flicker-noise temporal correlation spectrum of the liposomes leads to τ ≃ 0.5 s, comparing favorably with an intrinsic reaction-cycle time of about 0.4 s from enzymology.

Original language	English
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	109
Issue number	45
Pages (from-to)	18442-18446
Number of pages	5
ISSN	0027-8424
DOIs	https://doi.org/10.1073/pnas.1209909109
Publication status	Published - 2012
Externally published	Yes

Keywords

Membrane dynamics
Sodium pump reconstituted in giant vesicles
Vesicle fluctuation analysis

Access to Document

10.1073/pnas.1209909109

Cite this

Intrinsic reaction-cycle time scale of Na⁺,K⁺-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes. / Bouvrais, Hélène; Cornelius, Flemming; Ipsen, John H.; Mouritsen, Ole G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 45, 2012, p. 18442-18446.

Research output: Contribution to journal › Journal article › Research › peer-review

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AU - Ipsen, John H.

AU - Mouritsen, Ole G.

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