@inbook{da60bd67ece04b6fa9851621a6dbbaf7,
title = "Isolation of RNP granules",
abstract = "The post-transcriptional operon provides a means of synexpression of mRNAs encoding interrelated proteins. The coordination of gene expression may be achieved by a trans-acting RNA-binding protein attaching to similar cis-elements in different, yet functionally clustered, mRNAs. The RNP granule can be regarded as a supramolecular assembly of RNA and protein, probably representing several overlapping post-transcriptional operons. The present protocol describes how RNP granules may be isolated by the transgenic expression of a 3X FLAG version of an RNA-binding protein under tetracycline control via the tetracycline receptor/operator complex. In this way, inclusion of an appropriate tetracycline concentration ensures expression of the tagged version at the endogenous level, and the 3X FLAG tag is a convenient {"}handle{"} for the subsequent immunoprecipitation by immobilized anti-FLAG antibody.",
author = "Lars J{\o}nson and Nielsen, {Finn Cilius} and Jan Christiansen",
year = "2011",
doi = "10.1007/978-1-59745-248-9_18",
language = "English",
isbn = "978-1-58829-913-0",
volume = "703",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "265--273",
editor = "Henrik Nielsen",
booktitle = "RNA",
address = "Switzerland",
}