Large-scale benchmarking reveals false discoveries and count transformation sensitivity in 16S rRNA gene amplicon data analysis methods used in microbiome studies

Jonathan Thorsen; Asker Daniel Brejnrod; Martin Steen Mortensen; Morten Arendt Rasmussen; Jakob Stokholm; Waleed Abu Al-Soud; Søren Johannes Sørensen; Hans Bisgaard; Johannes Eichler Waage

doi:10.1186/s40168-016-0208-8

Large-scale benchmarking reveals false discoveries and count transformation sensitivity in 16S rRNA gene amplicon data analysis methods used in microbiome studies

Jonathan Thorsen, Asker Daniel Brejnrod, Martin Steen Mortensen, Morten Arendt Rasmussen, Jakob Stokholm, Waleed Abu Al-Soud, Søren Johannes Sørensen, Hans Bisgaard, Johannes Eichler Waage

Research output: Contribution to journal › Journal article › Research › peer-review

117 Citations (Scopus)

442 Downloads (Pure)

Original language	English
Article number	62
Journal	Microbiome
Volume	4
Number of pages	14
ISSN	2049-2618
DOIs	https://doi.org/10.1186/s40168-016-0208-8
Publication status	Published - 2016

Keywords

16S sequencing
Microbiome
Benchmark
Differential relative abundance
Beta-diversity

Access to Document

10.1186/s40168-016-0208-8Licence: CC BY

Thorsen_2016_Large-scale_benchmarkingFinal published version, 1.27 MBLicence: CC BY

Cite this

Thorsen, J., Brejnrod, A. D., Mortensen, M. S., Rasmussen, M. A., Stokholm, J., Abu Al-Soud, W., Sørensen, S. J., Bisgaard, H., & Waage, J. E. (2016). Large-scale benchmarking reveals false discoveries and count transformation sensitivity in 16S rRNA gene amplicon data analysis methods used in microbiome studies. Microbiome, 4, [62]. https://doi.org/10.1186/s40168-016-0208-8

Large-scale benchmarking reveals false discoveries and count transformation sensitivity in 16S rRNA gene amplicon data analysis methods used in microbiome studies. / Thorsen, Jonathan; Brejnrod, Asker Daniel; Mortensen, Martin Steen; Rasmussen, Morten Arendt ; Stokholm, Jakob; Abu Al-Soud, Waleed; Sørensen, Søren Johannes; Bisgaard, Hans; Waage, Johannes Eichler.

In: Microbiome, Vol. 4, 62, 2016.

Research output: Contribution to journal › Journal article › Research › peer-review

Thorsen, Jonathan ; Brejnrod, Asker Daniel ; Mortensen, Martin Steen ; Rasmussen, Morten Arendt ; Stokholm, Jakob ; Abu Al-Soud, Waleed ; Sørensen, Søren Johannes ; Bisgaard, Hans ; Waage, Johannes Eichler. / Large-scale benchmarking reveals false discoveries and count transformation sensitivity in 16S rRNA gene amplicon data analysis methods used in microbiome studies. In: Microbiome. 2016 ; Vol. 4.

@article{7c16c2ee215b401393965cec096b149e,

title = "Large-scale benchmarking reveals false discoveries and count transformation sensitivity in 16S rRNA gene amplicon data analysis methods used in microbiome studies",

keywords = "16S sequencing, Microbiome, Benchmark, Differential relative abundance, Beta-diversity",

author = "Jonathan Thorsen and Brejnrod, {Asker Daniel} and Mortensen, {Martin Steen} and Rasmussen, {Morten Arendt} and Jakob Stokholm and {Abu Al-Soud}, Waleed and S{\o}rensen, {S{\o}ren Johannes} and Hans Bisgaard and Waage, {Johannes Eichler}",

year = "2016",

doi = "10.1186/s40168-016-0208-8",

language = "English",

volume = "4",

journal = "Microbiome",

issn = "2049-2618",

publisher = "BioMed Central Ltd.",

}

TY - JOUR

T1 - Large-scale benchmarking reveals false discoveries and count transformation sensitivity in 16S rRNA gene amplicon data analysis methods used in microbiome studies

AU - Thorsen, Jonathan

AU - Brejnrod, Asker Daniel

AU - Mortensen, Martin Steen

AU - Rasmussen, Morten Arendt

AU - Stokholm, Jakob

AU - Abu Al-Soud, Waleed

AU - Sørensen, Søren Johannes

AU - Bisgaard, Hans

AU - Waage, Johannes Eichler

PY - 2016

Y1 - 2016

KW - 16S sequencing

KW - Microbiome

KW - Benchmark

KW - Differential relative abundance

KW - Beta-diversity

U2 - 10.1186/s40168-016-0208-8

DO - 10.1186/s40168-016-0208-8

M3 - Journal article

C2 - 27884206

VL - 4

JO - Microbiome

JF - Microbiome

SN - 2049-2618

M1 - 62

ER -