Abstract
Thrombosis is still a significant problem in microvascular surgery. The aim of this study was to evaluate the antithrombotic effect of topically applied active site-inhibited recombinant human factor VIIa (FFR-rFVIIa) in a rat model with microvascular thrombosis. Forty-five male rats were allocated to one of three groups: local treatment with vehicle only, local treatment with 0.035 mg of FFR-rFVIIa, or local treatment with 0.35 mg of FFR-rFVIIa. An arteriotomy was made in the right femoral artery. Ten minutes following topical application, a thrombogenic anastomosis was performed. Using a transilluminator, thrombus formation and anastomotic bleeding episodes were observed and registered for 40 min. Local application of FFR-rFVIIa resulted in a 85-90% reduction of thrombus formation in both treated groups compared to the control group, but the reduction was only statistically significant in the group treated with 0.035 mg of FFR-rFVIIa. An increased occurrence and duration of anastomotic bleeding episodes were observed in both FFR-rFVIIa-treated groups.
Original language | English |
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Journal | Microsurgery |
Volume | 19 |
Issue number | 8 |
Pages (from-to) | 369-73 |
Number of pages | 5 |
ISSN | 0738-1085 |
DOIs | |
Publication status | Published - 1999 |
Keywords
- Administration, Topical
- Animals
- Arteriovenous Anastomosis
- Factor VII/administration & dosage
- Factor VIIa
- Fibrinolytic Agents/administration & dosage
- Humans
- Male
- Microsurgery
- Postoperative Hemorrhage/etiology
- Rats
- Rats, Wistar
- Recombinant Proteins/administration & dosage
- Thrombosis/prevention & control