Long-term carriage and evolution of VREfmLong-term carriage and evolution of vancomycin-resistant Enterococcus faecium: A genomic study on consecutive isolates

Maja Johanne Sondergaard Knudsen; Jose Alfredo Samaniego Castruita; Sarah Mollerup; Barbara Juliane Holzknecht; Morten Hoppe; Henrik Westh; Mette Pinholt; Ingrid Maria Cecilia Rubin

doi:10.1093/jacamr/dlad153

Long-term carriage and evolution of VREfmLong-term carriage and evolution of vancomycin-resistant Enterococcus faecium: A genomic study on consecutive isolates

Maja Johanne Sondergaard Knudsen^*, Jose Alfredo Samaniego Castruita, Sarah Mollerup, Barbara Juliane Holzknecht, Morten Hoppe, Henrik Westh, Mette Pinholt, Ingrid Maria Cecilia Rubin

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Objectives: To determine if vancomycin-resistant Enterococcus faecium (VREfm) carriers carry the same VREfm clone after a minimum follow-up of 365days. For those carrying the same clone, we investigated the genomic evolution per year per genome. Methods: We used WGS results to assign VREfm clones to each isolate and determine clone shifts. Finally, we calculated distance in core-genome MLST alleles, and the number of SNPs between consecutive VREfm isolates from patients carrying the same VREfm clone. Results: In total, 44.2% of patients carried the same VREfm clone, and the genomic evolution was 1.8 alleles and 2.6 SNPs per genome per year. Conclusions: In our population of long-term carriers, we calculated a molecular clock of 2.6 SNPs.

Original language	English
Article number	dlad153
Journal	JAC-Antimicrobial Resistance
Volume	6
Issue number	1
Number of pages	4
ISSN	2632-1823
DOIs	https://doi.org/10.1093/jacamr/dlad153
Publication status	Published - 2024

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Publisher Copyright:
© 2023 The Author(s). Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy.

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Knudsen, M. J. S., Samaniego Castruita, J. A., Mollerup, S., Holzknecht, B. J., Hoppe, M., Westh, H., Pinholt, M., & Rubin, I. M. C. (2024). Long-term carriage and evolution of VREfmLong-term carriage and evolution of vancomycin-resistant Enterococcus faecium: A genomic study on consecutive isolates. JAC-Antimicrobial Resistance, 6(1), [dlad153]. https://doi.org/10.1093/jacamr/dlad153

Long-term carriage and evolution of VREfmLong-term carriage and evolution of vancomycin-resistant Enterococcus faecium : A genomic study on consecutive isolates. / Knudsen, Maja Johanne Sondergaard; Samaniego Castruita, Jose Alfredo; Mollerup, Sarah; Holzknecht, Barbara Juliane; Hoppe, Morten; Westh, Henrik; Pinholt, Mette; Rubin, Ingrid Maria Cecilia.

In: JAC-Antimicrobial Resistance, Vol. 6, No. 1, dlad153, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Knudsen, MJS, Samaniego Castruita, JA, Mollerup, S, Holzknecht, BJ, Hoppe, M, Westh, H, Pinholt, M & Rubin, IMC 2024, 'Long-term carriage and evolution of VREfmLong-term carriage and evolution of vancomycin-resistant Enterococcus faecium: A genomic study on consecutive isolates', JAC-Antimicrobial Resistance, vol. 6, no. 1, dlad153. https://doi.org/10.1093/jacamr/dlad153

Knudsen, Maja Johanne Sondergaard ; Samaniego Castruita, Jose Alfredo ; Mollerup, Sarah ; Holzknecht, Barbara Juliane ; Hoppe, Morten ; Westh, Henrik ; Pinholt, Mette ; Rubin, Ingrid Maria Cecilia. / Long-term carriage and evolution of VREfmLong-term carriage and evolution of vancomycin-resistant Enterococcus faecium : A genomic study on consecutive isolates. In: JAC-Antimicrobial Resistance. 2024 ; Vol. 6, No. 1.

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abstract = "Objectives: To determine if vancomycin-resistant Enterococcus faecium (VREfm) carriers carry the same VREfm clone after a minimum follow-up of 365days. For those carrying the same clone, we investigated the genomic evolution per year per genome. Methods: We used WGS results to assign VREfm clones to each isolate and determine clone shifts. Finally, we calculated distance in core-genome MLST alleles, and the number of SNPs between consecutive VREfm isolates from patients carrying the same VREfm clone. Results: In total, 44.2% of patients carried the same VREfm clone, and the genomic evolution was 1.8 alleles and 2.6 SNPs per genome per year. Conclusions: In our population of long-term carriers, we calculated a molecular clock of 2.6 SNPs.",

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doi = "10.1093/jacamr/dlad153",

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AU - Knudsen, Maja Johanne Sondergaard

AU - Samaniego Castruita, Jose Alfredo

AU - Mollerup, Sarah

AU - Holzknecht, Barbara Juliane

AU - Hoppe, Morten

AU - Westh, Henrik

AU - Pinholt, Mette

AU - Rubin, Ingrid Maria Cecilia

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AB - Objectives: To determine if vancomycin-resistant Enterococcus faecium (VREfm) carriers carry the same VREfm clone after a minimum follow-up of 365days. For those carrying the same clone, we investigated the genomic evolution per year per genome. Methods: We used WGS results to assign VREfm clones to each isolate and determine clone shifts. Finally, we calculated distance in core-genome MLST alleles, and the number of SNPs between consecutive VREfm isolates from patients carrying the same VREfm clone. Results: In total, 44.2% of patients carried the same VREfm clone, and the genomic evolution was 1.8 alleles and 2.6 SNPs per genome per year. Conclusions: In our population of long-term carriers, we calculated a molecular clock of 2.6 SNPs.

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