Lysine acetylation targets protein complexes and co-regulates major cellular functions

Chuna Ram Choudhary, Chanchal Kumar, Florian Gnad, Michael L Nielsen, Michael Rehman, Tobias C Walther, Jesper V Olsen, Matthias Mann

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3409 Citations (Scopus)

Abstract

Lysine acetylation is a reversible posttranslational modification of proteins and plays a key role in regulating gene expression. Technological limitations have so far prevented a global analysis of lysine acetylation's cellular roles. We used high-resolution mass spectrometry to identify 3600 lysine acetylation sites on 1750 proteins and quantified acetylation changes in response to the deacetylase inhibitors suberoylanilide hydroxamic acid and MS-275. Lysine acetylation preferentially targets large macromolecular complexes involved in diverse cellular processes, such as chromatin remodeling, cell cycle, splicing, nuclear transport, and actin nucleation. Acetylation impaired phosphorylation-dependent interactions of 14-3-3 and regulated the yeast cyclin-dependent kinase Cdc28. Our data demonstrate that the regulatory scope of lysine acetylation is broad and comparable with that of other major posttranslational modifications.
Original languageEnglish
JournalScience
Volume325
Issue number5942
Pages (from-to)834-40
Number of pages6
ISSN0036-8075
DOIs
Publication statusPublished - 2009

Bibliographical note

Keywords: Acetylation; Amino Acid Motifs; Benzamides; Cell Line, Tumor; Cell Nucleus; Cell Physiological Phenomena; Cytoplasm; Enzyme Inhibitors; Histone Deacetylases; Humans; Hydroxamic Acids; Lysine; Mass Spectrometry; Metabolic Networks and Pathways; Mitochondria; Multiprotein Complexes; Protein Processing, Post-Translational; Protein Structure, Tertiary; Proteins; Proteome; Proteomics; Pyridines; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins

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