Measuring Histamine and Cytokine Release from Basophils and Mast Cells

Bettina M. Jensen; Ewa Anna Bartko; Katrine Baumann; Per Stahl Skov

doi:10.1007/978-1-0716-0696-4_21

Measuring Histamine and Cytokine Release from Basophils and Mast Cells

Bettina M. Jensen^*, Ewa Anna Bartko, Katrine Baumann, Per Stahl Skov

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

9 Citations (Scopus)

Abstract

Basophils and mast cells are known for their capability to release both preformed and newly synthesized inflammatory mediators. In this chapter, we describe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells (the latter by microdialysis), using either a solid phase assay or flow cytometry. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detection.

Original language	English
Title of host publication	Basophils and Mast Cells
Publisher	Springer
Publication date	7 Aug 2020
ISBN (Print)	9781071606957, 9781071606964
DOIs	https://doi.org/10.1007/978-1-0716-0696-4_21
Publication status	Published - 7 Aug 2020
Externally published	Yes

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10.1007/978-1-0716-0696-4_21

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Cite this

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