Abstract
Plant DNA barcoding has a multitude of applications ranging from species detection and biomonitoring to investigating ecological networks and checking food quality. The ability to accurately identify species, using DNA barcoding, depends on the quality and comprehensiveness of the reference library that is used. This chapter describes how to create plant reference libraries using the rbcL, matK, and ITS2 DNA barcode regions. It covers the creation of species lists, the collection of specimens from the field and herbarium, DNA extraction, PCR amplification, and DNA sequencing. This methodology gives special attention to using samples from herbaria, as they represent important collections of easily accessible, taxonomically verified plant material.
| Original language | English |
|---|---|
| Title of host publication | DNA Barcoding : Methods and Protocols |
| Editors | Robert DeSalle |
| Number of pages | 29 |
| Publisher | Humana Press |
| Publication date | 2024 |
| Pages | 445-473 |
| ISBN (Print) | 978-1-0716-3580-3 |
| ISBN (Electronic) | 978-1-0716-3581-0 |
| DOIs | |
| Publication status | Published - 2024 |
| Series | Methods in Molecular Biology |
|---|---|
| ISSN | 1064-3745 |
Bibliographical note
Publisher Copyright:© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
Keywords
- Digitization
- DNA barcoding
- DNA extraction
- Extended specimen collection
- Herbarium specimens
- ITS2
- matK
- Open access
- PCR
- Plant collection
- Plants
- rbcL
- Species identification