Abstract
To quantify known and unknown microorganisms at species-level resolution using shotgun sequencing data, we developed a method that establishes metagenomic operational taxonomic units (mOTUs) based on single-copy phylogenetic marker genes. Applied to 252 human fecal samples, the method revealed that on average 43% of the species abundance and 58% of the richness cannot be captured by current reference genome-based methods. An implementation of the method is available at http://www.bork.embl.de/software/mOTU/.
Original language | English |
---|---|
Journal | Nature Methods |
Volume | 10 |
Issue number | 12 |
Pages (from-to) | 1196-1199 |
Number of pages | 4 |
ISSN | 1548-7091 |
DOIs | |
Publication status | Published - 2013 |