Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni

Yilmaz Emre Gencay*, Tina Birk, Martine Camilla Holst Sørensen, Lone Brøndsted

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

37 Citations (Scopus)

Abstract

Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.

Original languageEnglish
Title of host publicationCampylobacter jejuni : Methods and Protocols
Number of pages10
Volume1512
PublisherHumana Press
Publication date2017
Pages19-28
ISBN (Print)978-1-4939-6534-2
ISBN (Electronic)978-1-4939-6536-6
DOIs
Publication statusPublished - 2017
SeriesMethods in Molecular Biology
Volume1512
ISSN1064-3745

Keywords

  • Bacteriophage
  • C. jejuni
  • Campylobacter
  • Isolation
  • Propagation
  • Purification
  • Titration

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