NADPH oxidase is internalized by clathrin-coated pits and localizes to a Rab27A/B GTPase-regulated secretory compartment in activated macrophages

Patrick Ejlerskov; Dan Ploug Christensen; David Beyaie; James B Burritt; Marie-Helene Paclet; Agnes Gorlach; Bo van Deurs; Frederik Vilhardt

doi:10.1074/jbc.M111.293696

NADPH oxidase is internalized by clathrin-coated pits and localizes to a Rab27A/B GTPase-regulated secretory compartment in activated macrophages

Patrick Ejlerskov, Dan Ploug Christensen, David Beyaie, James B Burritt, Marie-Helene Paclet, Agnes Gorlach, Bo van Deurs, Frederik Vilhardt

Research output: Contribution to journal › Journal article › Research › peer-review

30 Citations (Scopus)

Abstract

Here, we report that activation of different types of tissue macrophages, including microglia, by lipopolysaccharide (LPS) or GM-CSF stimulation correlates with the quantitative redistribution of NADPH oxidase (cyt b(558)) from the plasma membrane to an intracellular stimulus-responsive storage compartment. Cryo-immunogold labeling of gp91(phox) and CeCl(3) cytochemistry showed the presence of gp91(phox) and oxidant production in numerous small (

Original language	English
Journal	Journal of Biological Chemistry
Volume	287
Issue number	7
Pages (from-to)	4835-52
Number of pages	18
ISSN	0021-9258
DOIs	https://doi.org/10.1074/jbc.M111.293696
Publication status	Published - Feb 2012

Keywords

Adenosine Triphosphate
Animals
CD40 Ligand
Calcium-Binding Proteins
Cells, Cultured
Clathrin
Clathrin-Coated Vesicles
Cytochrome b Group
Endosomes
Exocytosis
Intracellular Signaling Peptides and Proteins
Macrophage Activation
Macrophages
Membrane Glycoproteins
Microglia
NADPH Oxidase
Rats
Superoxides
Tumor Necrosis Factor-alpha
rab GTP-Binding Proteins

Access to Document

10.1074/jbc.M111.293696

Cite this

NADPH oxidase is internalized by clathrin-coated pits and localizes to a Rab27A/B GTPase-regulated secretory compartment in activated macrophages. / Ejlerskov, Patrick; Christensen, Dan Ploug; Beyaie, David; Burritt, James B; Paclet, Marie-Helene; Gorlach, Agnes; van Deurs, Bo; Vilhardt, Frederik.

In: Journal of Biological Chemistry, Vol. 287, No. 7, 02.2012, p. 4835-52.

Research output: Contribution to journal › Journal article › Research › peer-review

Ejlerskov, Patrick ; Christensen, Dan Ploug ; Beyaie, David ; Burritt, James B ; Paclet, Marie-Helene ; Gorlach, Agnes ; van Deurs, Bo ; Vilhardt, Frederik. / NADPH oxidase is internalized by clathrin-coated pits and localizes to a Rab27A/B GTPase-regulated secretory compartment in activated macrophages. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 7. pp. 4835-52.

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abstract = "Here, we report that activation of different types of tissue macrophages, including microglia, by lipopolysaccharide (LPS) or GM-CSF stimulation correlates with the quantitative redistribution of NADPH oxidase (cyt b(558)) from the plasma membrane to an intracellular stimulus-responsive storage compartment. Cryo-immunogold labeling of gp91(phox) and CeCl(3) cytochemistry showed the presence of gp91(phox) and oxidant production in numerous small (",

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AU - Ejlerskov, Patrick

AU - Christensen, Dan Ploug

AU - Beyaie, David

AU - Burritt, James B

AU - Paclet, Marie-Helene

AU - Gorlach, Agnes

AU - van Deurs, Bo

AU - Vilhardt, Frederik

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AB - Here, we report that activation of different types of tissue macrophages, including microglia, by lipopolysaccharide (LPS) or GM-CSF stimulation correlates with the quantitative redistribution of NADPH oxidase (cyt b(558)) from the plasma membrane to an intracellular stimulus-responsive storage compartment. Cryo-immunogold labeling of gp91(phox) and CeCl(3) cytochemistry showed the presence of gp91(phox) and oxidant production in numerous small (

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KW - Animals

KW - CD40 Ligand

KW - Calcium-Binding Proteins

KW - Cells, Cultured

KW - Clathrin

KW - Clathrin-Coated Vesicles

KW - Cytochrome b Group

KW - Endosomes

KW - Exocytosis

KW - Intracellular Signaling Peptides and Proteins

KW - Macrophage Activation

KW - Macrophages

KW - Membrane Glycoproteins

KW - Microglia

KW - NADPH Oxidase

KW - Rats

KW - Superoxides

KW - Tumor Necrosis Factor-alpha

KW - rab GTP-Binding Proteins

U2 - 10.1074/jbc.M111.293696

DO - 10.1074/jbc.M111.293696

M3 - Journal article

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VL - 287

SP - 4835

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JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

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ER -