New advanced glycation end products observed in rat urine by untargeted metabolomics after feeding with heat-treated skimmed milk powder

Xiaomin Zhou; Maria M Ulaszewska; Cristian De Gobba; Åsmund Rinnan; Malene Wibe Poulsen; Jie Chen; Fulvio Mattivi; Rikke Vingborg Hedegaard; Leif Horsfelt Skibsted; Lars Ove Dragsted

doi:10.1002/mnfr.202001049

New advanced glycation end products observed in rat urine by untargeted metabolomics after feeding with heat-treated skimmed milk powder

Xiaomin Zhou, Maria M Ulaszewska, Cristian De Gobba, Åsmund Rinnan, Malene Wibe Poulsen, Jie Chen, Fulvio Mattivi, Rikke Vingborg Hedegaard, Leif Horsfelt Skibsted, Lars Ove Dragsted^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

3 Citations (Scopus)

Abstract

Scope: Milk powder is commonly consumed throughout the world. However, advanced glycation end products (AGEs) will form in milk powder during thermal processing and long-term storage. We aimed to identify such compounds with potential as new urinary biomarkers of intake of heat-treated skimmed milk powder (HSMP).

Methods and results: A parallel study was performed with different dosages of HSMP as well as hydrolyzed HSMP and untreated skimmed milk powder (SMP) in 36 rats. The 24-h urine samples on day 7 or 8 were collected and profiled by untargeted UPLC-Qtof-MS metabolomics. Statistical analysis revealed 25 metabolites differentiating SMP and HSMP; nineteen of these structures were proposed as lysine- and arginine- derived AGEs, and heterocyclic compounds.

Conclusion: These metabolites may potentially serve as biomarkers of food intake pending further validation to assess intakes of heat-processed dairy foods and thus help to elucidate the effects of HSMP consumption or dietary AGEs on human health.

Original language	English
Article number	2001049
Journal	Molecular Nutrition & Food Research
Volume	65
Issue number	7
Number of pages	9
ISSN	1613-4125
DOIs	https://doi.org/10.1002/mnfr.202001049
Publication status	Published - 2021

Bibliographical note

Keywords

Faculty of Science
Skimmed milk powder
Metabolomics
Advanced glycation end products
Maillard reaction
Biomarker

Access to Document

10.1002/mnfr.202001049

Cite this

Zhou, X., Ulaszewska, M. M., De Gobba, C., Rinnan, Å., Poulsen, M. W., Chen, J., Mattivi, F., Hedegaard, R. V., Skibsted, L. H., & Dragsted, L. O. (2021). New advanced glycation end products observed in rat urine by untargeted metabolomics after feeding with heat-treated skimmed milk powder. Molecular Nutrition & Food Research, 65(7), [2001049]. https://doi.org/10.1002/mnfr.202001049

New advanced glycation end products observed in rat urine by untargeted metabolomics after feeding with heat-treated skimmed milk powder. / Zhou, Xiaomin; Ulaszewska, Maria M; De Gobba, Cristian ; Rinnan, Åsmund; Poulsen, Malene Wibe; Chen, Jie; Mattivi, Fulvio; Hedegaard, Rikke Vingborg; Skibsted, Leif Horsfelt ; Dragsted, Lars Ove.

In: Molecular Nutrition & Food Research, Vol. 65, No. 7, 2001049, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Zhou, X, Ulaszewska, MM, De Gobba, C , Rinnan, Å, Poulsen, MW, Chen, J, Mattivi, F, Hedegaard, RV, Skibsted, LH & Dragsted, LO 2021, 'New advanced glycation end products observed in rat urine by untargeted metabolomics after feeding with heat-treated skimmed milk powder', Molecular Nutrition & Food Research, vol. 65, no. 7, 2001049. https://doi.org/10.1002/mnfr.202001049

Zhou, Xiaomin ; Ulaszewska, Maria M ; De Gobba, Cristian ; Rinnan, Åsmund ; Poulsen, Malene Wibe ; Chen, Jie ; Mattivi, Fulvio ; Hedegaard, Rikke Vingborg ; Skibsted, Leif Horsfelt ; Dragsted, Lars Ove. / New advanced glycation end products observed in rat urine by untargeted metabolomics after feeding with heat-treated skimmed milk powder. In: Molecular Nutrition & Food Research. 2021 ; Vol. 65, No. 7.

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abstract = "Scope: Milk powder is commonly consumed throughout the world. However, advanced glycation end products (AGEs) will form in milk powder during thermal processing and long-term storage. We aimed to identify such compounds with potential as new urinary biomarkers of intake of heat-treated skimmed milk powder (HSMP).Methods and results: A parallel study was performed with different dosages of HSMP as well as hydrolyzed HSMP and untreated skimmed milk powder (SMP) in 36 rats. The 24-h urine samples on day 7 or 8 were collected and profiled by untargeted UPLC-Qtof-MS metabolomics. Statistical analysis revealed 25 metabolites differentiating SMP and HSMP; nineteen of these structures were proposed as lysine- and arginine- derived AGEs, and heterocyclic compounds.Conclusion: These metabolites may potentially serve as biomarkers of food intake pending further validation to assess intakes of heat-processed dairy foods and thus help to elucidate the effects of HSMP consumption or dietary AGEs on human health.",

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AU - Zhou, Xiaomin

AU - Ulaszewska, Maria M

AU - De Gobba, Cristian

AU - Rinnan, Åsmund

AU - Poulsen, Malene Wibe

AU - Chen, Jie

AU - Mattivi, Fulvio

AU - Hedegaard, Rikke Vingborg

AU - Skibsted, Leif Horsfelt

AU - Dragsted, Lars Ove

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N2 - Scope: Milk powder is commonly consumed throughout the world. However, advanced glycation end products (AGEs) will form in milk powder during thermal processing and long-term storage. We aimed to identify such compounds with potential as new urinary biomarkers of intake of heat-treated skimmed milk powder (HSMP).Methods and results: A parallel study was performed with different dosages of HSMP as well as hydrolyzed HSMP and untreated skimmed milk powder (SMP) in 36 rats. The 24-h urine samples on day 7 or 8 were collected and profiled by untargeted UPLC-Qtof-MS metabolomics. Statistical analysis revealed 25 metabolites differentiating SMP and HSMP; nineteen of these structures were proposed as lysine- and arginine- derived AGEs, and heterocyclic compounds.Conclusion: These metabolites may potentially serve as biomarkers of food intake pending further validation to assess intakes of heat-processed dairy foods and thus help to elucidate the effects of HSMP consumption or dietary AGEs on human health.

AB - Scope: Milk powder is commonly consumed throughout the world. However, advanced glycation end products (AGEs) will form in milk powder during thermal processing and long-term storage. We aimed to identify such compounds with potential as new urinary biomarkers of intake of heat-treated skimmed milk powder (HSMP).Methods and results: A parallel study was performed with different dosages of HSMP as well as hydrolyzed HSMP and untreated skimmed milk powder (SMP) in 36 rats. The 24-h urine samples on day 7 or 8 were collected and profiled by untargeted UPLC-Qtof-MS metabolomics. Statistical analysis revealed 25 metabolites differentiating SMP and HSMP; nineteen of these structures were proposed as lysine- and arginine- derived AGEs, and heterocyclic compounds.Conclusion: These metabolites may potentially serve as biomarkers of food intake pending further validation to assess intakes of heat-processed dairy foods and thus help to elucidate the effects of HSMP consumption or dietary AGEs on human health.

KW - Faculty of Science

KW - Skimmed milk powder

KW - Metabolomics

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KW - Maillard reaction

KW - Biomarker

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