Nisin-loaded chitosan/sodium alginate microspheres enhance the antimicrobial efficacy of nisin against Staphylococcus aureus

Taya Tang; Yinzhu Chen; Zhongling Zhao; Qianyu Bai; Jørgen J. Leisner; Tianlong Liu

doi:10.1093/jambio/lxae259

Nisin-loaded chitosan/sodium alginate microspheres enhance the antimicrobial efficacy of nisin against Staphylococcus aureus

Taya Tang, Yinzhu Chen, Zhongling Zhao, Qianyu Bai, Jørgen J. Leisner, Tianlong Liu^*

^*Corresponding author for this work

Food Safety and Zoonoses

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Aims: To develop and evaluate nisin-loaded chitosan/sodium alginate (CS/SA) microspheres as an improved antimicrobial delivery system targeting Staphylococcus aureus strains. Methods and results: The microspheres were prepared using a modified water-in-oil emulsion cross-linking method, resulting in spherical particles sized 1-8 μm with a surface charge of -7.92 ± 5.09 mV, confirmed by scanning electron microscopy (SEM) and Zetasizer analysis. Encapsulation efficiency (EE) and loading capacity (LC) of nisin were 87.60% ± 0.43% and 1.99% ± 0.01%, respectively. In vitro release studies over 48 h indicated a controlled release pattern of nisin, described by the Korsmeyer-Peppas model, with higher release rates at 37°C and alkaline pH. Antimicrobial assays showed an enhanced efficacy of nisin-loaded CS/SA microspheres compared to free nisin, with minimum inhibitory concentration values reduced by 50%. Confocal laser scanning microscopy (CLSM), SEM, and transmission electron microscopy showed significant bacterial membrane damage and cellular disruption induced by the microspheres. Conclusions: This study highlights the potential of nisin-loaded CS/SA microspheres as an innovative antimicrobial delivery system with improved stability and antimicrobial efficacy against S. aureus, addressing limitations associated with nisin applied alone.

Original language	English
Article number	lxae259
Journal	Journal of Applied Microbiology
Volume	135
Issue number	11
Number of pages	15
ISSN	1364-5072
DOIs	https://doi.org/10.1093/jambio/lxae259
Publication status	Published - 2024

Bibliographical note

Funding Information:
This work was funded by a PhD study grant to TT from China Scholarship Council (CSC No. 202006350030), a research grant to JJL from the Norwegian Research Council (grant FRIPRO 314490), and funding support to TL from the Young Teachers Innovation Project of China Agricultural University (Project No. 2018QC142) and the National Natural Science Foundation of China (Project No. 31802162).

Publisher Copyright:
© 2024 The Author(s). Published by Oxford University Press on behalf of Applied Microbiology International.

Keywords

antimicrobial activity
chitosan/sodium alginate microspheres
controlled release
foodborne diseases
nisin
Staphylococcus aureus

Access to Document

10.1093/jambio/lxae259

Cite this

Nisin-loaded chitosan/sodium alginate microspheres enhance the antimicrobial efficacy of nisin against Staphylococcus aureus. / Tang, Taya; Chen, Yinzhu; Zhao, Zhongling; Bai, Qianyu; Leisner, Jørgen J.; Liu, Tianlong.

In: Journal of Applied Microbiology, Vol. 135, No. 11, lxae259, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Nisin-loaded chitosan/sodium alginate microspheres enhance the antimicrobial efficacy of nisin against Staphylococcus aureus",

abstract = "Aims: To develop and evaluate nisin-loaded chitosan/sodium alginate (CS/SA) microspheres as an improved antimicrobial delivery system targeting Staphylococcus aureus strains. Methods and results: The microspheres were prepared using a modified water-in-oil emulsion cross-linking method, resulting in spherical particles sized 1-8 μm with a surface charge of -7.92 ± 5.09 mV, confirmed by scanning electron microscopy (SEM) and Zetasizer analysis. Encapsulation efficiency (EE) and loading capacity (LC) of nisin were 87.60% ± 0.43% and 1.99% ± 0.01%, respectively. In vitro release studies over 48 h indicated a controlled release pattern of nisin, described by the Korsmeyer-Peppas model, with higher release rates at 37°C and alkaline pH. Antimicrobial assays showed an enhanced efficacy of nisin-loaded CS/SA microspheres compared to free nisin, with minimum inhibitory concentration values reduced by 50%. Confocal laser scanning microscopy (CLSM), SEM, and transmission electron microscopy showed significant bacterial membrane damage and cellular disruption induced by the microspheres. Conclusions: This study highlights the potential of nisin-loaded CS/SA microspheres as an innovative antimicrobial delivery system with improved stability and antimicrobial efficacy against S. aureus, addressing limitations associated with nisin applied alone. ",

keywords = "antimicrobial activity, chitosan/sodium alginate microspheres, controlled release, foodborne diseases, nisin, Staphylococcus aureus",

author = "Taya Tang and Yinzhu Chen and Zhongling Zhao and Qianyu Bai and Leisner, {J{\o}rgen J.} and Tianlong Liu",

note = "Funding Information: This work was funded by a PhD study grant to TT from China Scholarship Council (CSC No. 202006350030), a research grant to JJL from the Norwegian Research Council (grant FRIPRO 314490), and funding support to TL from the Young Teachers Innovation Project of China Agricultural University (Project No. 2018QC142) and the National Natural Science Foundation of China (Project No. 31802162). Publisher Copyright: {\textcopyright} 2024 The Author(s). Published by Oxford University Press on behalf of Applied Microbiology International.",

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doi = "10.1093/jambio/lxae259",

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T1 - Nisin-loaded chitosan/sodium alginate microspheres enhance the antimicrobial efficacy of nisin against Staphylococcus aureus

AU - Tang, Taya

AU - Chen, Yinzhu

AU - Zhao, Zhongling

AU - Bai, Qianyu

AU - Leisner, Jørgen J.

AU - Liu, Tianlong

N1 - Funding Information: This work was funded by a PhD study grant to TT from China Scholarship Council (CSC No. 202006350030), a research grant to JJL from the Norwegian Research Council (grant FRIPRO 314490), and funding support to TL from the Young Teachers Innovation Project of China Agricultural University (Project No. 2018QC142) and the National Natural Science Foundation of China (Project No. 31802162). Publisher Copyright: © 2024 The Author(s). Published by Oxford University Press on behalf of Applied Microbiology International.

PY - 2024

Y1 - 2024

N2 - Aims: To develop and evaluate nisin-loaded chitosan/sodium alginate (CS/SA) microspheres as an improved antimicrobial delivery system targeting Staphylococcus aureus strains. Methods and results: The microspheres were prepared using a modified water-in-oil emulsion cross-linking method, resulting in spherical particles sized 1-8 μm with a surface charge of -7.92 ± 5.09 mV, confirmed by scanning electron microscopy (SEM) and Zetasizer analysis. Encapsulation efficiency (EE) and loading capacity (LC) of nisin were 87.60% ± 0.43% and 1.99% ± 0.01%, respectively. In vitro release studies over 48 h indicated a controlled release pattern of nisin, described by the Korsmeyer-Peppas model, with higher release rates at 37°C and alkaline pH. Antimicrobial assays showed an enhanced efficacy of nisin-loaded CS/SA microspheres compared to free nisin, with minimum inhibitory concentration values reduced by 50%. Confocal laser scanning microscopy (CLSM), SEM, and transmission electron microscopy showed significant bacterial membrane damage and cellular disruption induced by the microspheres. Conclusions: This study highlights the potential of nisin-loaded CS/SA microspheres as an innovative antimicrobial delivery system with improved stability and antimicrobial efficacy against S. aureus, addressing limitations associated with nisin applied alone.

AB - Aims: To develop and evaluate nisin-loaded chitosan/sodium alginate (CS/SA) microspheres as an improved antimicrobial delivery system targeting Staphylococcus aureus strains. Methods and results: The microspheres were prepared using a modified water-in-oil emulsion cross-linking method, resulting in spherical particles sized 1-8 μm with a surface charge of -7.92 ± 5.09 mV, confirmed by scanning electron microscopy (SEM) and Zetasizer analysis. Encapsulation efficiency (EE) and loading capacity (LC) of nisin were 87.60% ± 0.43% and 1.99% ± 0.01%, respectively. In vitro release studies over 48 h indicated a controlled release pattern of nisin, described by the Korsmeyer-Peppas model, with higher release rates at 37°C and alkaline pH. Antimicrobial assays showed an enhanced efficacy of nisin-loaded CS/SA microspheres compared to free nisin, with minimum inhibitory concentration values reduced by 50%. Confocal laser scanning microscopy (CLSM), SEM, and transmission electron microscopy showed significant bacterial membrane damage and cellular disruption induced by the microspheres. Conclusions: This study highlights the potential of nisin-loaded CS/SA microspheres as an innovative antimicrobial delivery system with improved stability and antimicrobial efficacy against S. aureus, addressing limitations associated with nisin applied alone.

KW - antimicrobial activity

KW - chitosan/sodium alginate microspheres

KW - controlled release

KW - foodborne diseases

KW - nisin

KW - Staphylococcus aureus

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DO - 10.1093/jambio/lxae259

M3 - Journal article

C2 - 39394666

AN - SCOPUS:85208514796

VL - 135

JO - Proceedings of the Society for Applied Bacteriology

JF - Proceedings of the Society for Applied Bacteriology

SN - 0266-8254

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ER -