Occurrence of Salmonella enterica in faecal sludge from Nigeria and genetic relatedness with strains associated with human infections in Africa

Abdurrahman Hassan Jibril, Anders Dalsgaard, Iruka N. Okeke, Aliyu Musawa Ibrahim, John Elmerdahl Olsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

1 Citation (Scopus)

Abstract

Aims: This study investigated occurrence of Salmonella in faecal sludge from public toilets in Nigeria and genetic relatedness of strains that have been reported to cause human infection across Africa. Methods and results: The study collected 150 human sludge from public toilets and identified Salmonella through culture and PCR. Isolates were tested for antimicrobial susceptibility and sequenced using Illumina MiSeq. Draft sequences were compared with sequence data from Enterobase and GenBank. Twenty-four (16.0%) of sewage samples were positive for Salmonella [CI95 (10.2-21.8)]. Salmonella serotype Give [sequence type (ST) 516], Salmonella serotype Seftenberg (ST-14), and Salmonella serotype Chester (ST-411) were the most prevalent serovars found in 45.8%, 16.7%, and 16.7% of samples, respectively. Most of the isolates were sensitive to the antimicrobials tested, only one isolate of Salmonella serotype Derby showed resistance to ampicillin and cefazolin. Notably, 91.7% of the strains had the aac (6)-Iaa gene and point mutations in parC, gyrA, and acrB. Salmonella serotype Chester showed genetic relatedness with strains from Benin Republic and South Africa. Conclusions: There is genetic relatedness of present strains and those associated with human infections in Africa.

Original languageEnglish
Article numberlxae293
JournalJournal of Applied Microbiology
Volume135
Issue number12
Number of pages8
ISSN1364-5072
DOIs
Publication statusPublished - 2024

Bibliographical note

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© 2024 The Author(s).

Keywords

  • antibiotic resistance
  • faecal sludge
  • genetic relatedness
  • Nigeria
  • Salmonella
  • sequence

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