TY - JOUR
T1 - Optimized Green Extraction of Polyphenols from Cassia javanica L. Petals for Their Application in Sunflower Oil
T2 - Anticancer and Antioxidant Properties
AU - Younis, Mohamed Ibrahim
AU - Xiaofeng, Ren
AU - Alzubaidi, Azalldeen Kazal
AU - Mahmoud, Khaled Fahmy
AU - Altemimi, Ammar B.
AU - Cacciola, Francesco
AU - Raza, Husnain
AU - Pratap-Singh, Anubhav
AU - Abedelmaksoud, Tarek Gamal
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/7/1
Y1 - 2022/7/1
N2 - The total phenolic content (TPC) from Cassia javanica L. petals were extracted using ethano-lic solvent extraction at concentrations ranging from 0 to 90% and an SCF-CO2 co-solvent at various pressures. Ultrasound-assisted extraction parameters were optimized using response surface method-ology (RSM). Antioxidant and anticancer properties of total phenols were assessed. An SCF-CO2 co-solvent extract was nano-encapsulated and applied to sunflower oil without the addition of an antioxidant. The results indicated that the best treatment for retaining TPC and total flavonoids content (TFC) was SCF-CO2 co-solvent followed by the ultrasound and ethanolic extraction pro-cedures. Additionally, the best antioxidant activity by β-carotene/linoleic acid and DPPH free radical-scavenging test systems was observed by SCF-CO2 co-solvent then ultrasound and ethanolic extraction methods. SCF-CO2 co-solvent recorded the highest inhibition % for PC3 (76.20%) and MCF7 (98.70%) and the lowest IC50 value for PC3 (145 µ/mL) and MCF7 (96 µ/mL). It was discovered that fortifying sunflower oil with SCF-CO2 co-solvent nanoparticles had a beneficial effect on free fatty acids and peroxide levels. The SCF-CO2 method was finally found to be superior and could be used in large-scale processing.
AB - The total phenolic content (TPC) from Cassia javanica L. petals were extracted using ethano-lic solvent extraction at concentrations ranging from 0 to 90% and an SCF-CO2 co-solvent at various pressures. Ultrasound-assisted extraction parameters were optimized using response surface method-ology (RSM). Antioxidant and anticancer properties of total phenols were assessed. An SCF-CO2 co-solvent extract was nano-encapsulated and applied to sunflower oil without the addition of an antioxidant. The results indicated that the best treatment for retaining TPC and total flavonoids content (TFC) was SCF-CO2 co-solvent followed by the ultrasound and ethanolic extraction pro-cedures. Additionally, the best antioxidant activity by β-carotene/linoleic acid and DPPH free radical-scavenging test systems was observed by SCF-CO2 co-solvent then ultrasound and ethanolic extraction methods. SCF-CO2 co-solvent recorded the highest inhibition % for PC3 (76.20%) and MCF7 (98.70%) and the lowest IC50 value for PC3 (145 µ/mL) and MCF7 (96 µ/mL). It was discovered that fortifying sunflower oil with SCF-CO2 co-solvent nanoparticles had a beneficial effect on free fatty acids and peroxide levels. The SCF-CO2 method was finally found to be superior and could be used in large-scale processing.
KW - antioxidant
KW - Cassia javanica
KW - nano-encapsulated
KW - SCF-CO
KW - β-carotene/linoleic acid
UR - http://www.scopus.com/inward/record.url?scp=85134028926&partnerID=8YFLogxK
U2 - 10.3390/molecules27144329
DO - 10.3390/molecules27144329
M3 - Journal article
C2 - 35889202
AN - SCOPUS:85134028926
VL - 27
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 14
M1 - 4329
ER -