Abstract
There is a significant interest in identifying blood-borne factors that mediate tissue crosstalk and function as molecular effectors of physical activity. Although past studies have focused on an individual molecule or cell type, the organism-wide secretome response to physical activity has not been evaluated. Here, we use a cell-type-specific proteomic approach to generate a 21-cell-type, 10-tissue map of exercise training-regulated secretomes in mice. Our dataset identifies >200 exercise training-regulated cell-type-secreted protein pairs, the majority of which have not been previously reported. Pdgfra-cre-labeled secretomes were the most responsive to exercise training. Finally, we show anti-obesity, anti-diabetic, and exercise performance-enhancing activities for proteoforms of intracellular carboxylesterases whose secretion from the liver is induced by exercise training.
Original language | English |
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Article number | e11 |
Journal | Cell Metabolism |
Volume | 35 |
Issue number | 7 |
Pages (from-to) | 1261-1279 |
Number of pages | 19 |
ISSN | 1550-4131 |
DOIs | |
Publication status | Published - 2023 |
Bibliographical note
Publisher Copyright:© 2023 Elsevier Inc.
Keywords
- cell type
- CES2
- energy metabolism
- exercise
- exercise performance
- hepatokine
- obesity
- secretome
- tissue crosstalk