Organism-wide, cell-type-specific secretome mapping of exercise training in mice

Wei Wei, Nicholas M. Riley, Xuchao Lyu, Xiaotao Shen, Jing Guo, Steffen H. Raun, Meng Zhao, Maria Dolores Moya-Garzon, Himanish Basu, Alan Sheng-Hwa Tung, Veronica L. Li, Wentao Huang, Amanda L. Wiggenhorn, Katrin J. Svensson, Michael P. Snyder, Carolyn R. Bertozzi, Jonathan Z. Long*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

32 Citations (Scopus)
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Abstract

There is a significant interest in identifying blood-borne factors that mediate tissue crosstalk and function as molecular effectors of physical activity. Although past studies have focused on an individual molecule or cell type, the organism-wide secretome response to physical activity has not been evaluated. Here, we use a cell-type-specific proteomic approach to generate a 21-cell-type, 10-tissue map of exercise training-regulated secretomes in mice. Our dataset identifies >200 exercise training-regulated cell-type-secreted protein pairs, the majority of which have not been previously reported. Pdgfra-cre-labeled secretomes were the most responsive to exercise training. Finally, we show anti-obesity, anti-diabetic, and exercise performance-enhancing activities for proteoforms of intracellular carboxylesterases whose secretion from the liver is induced by exercise training.

Original languageEnglish
Article numbere11
JournalCell Metabolism
Volume35
Issue number7
Pages (from-to)1261-1279
Number of pages19
ISSN1550-4131
DOIs
Publication statusPublished - 2023

Bibliographical note

Publisher Copyright:
© 2023 Elsevier Inc.

Keywords

  • cell type
  • CES2
  • energy metabolism
  • exercise
  • exercise performance
  • hepatokine
  • obesity
  • secretome
  • tissue crosstalk

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