Oxygen-tolerant, eosin Y mediated synthesis of protein-polymer biohybrids and protein-coated polymer nanoparticles

Errika Voutyritsa; Thomai Lazou; Jonida Bushi; Stavroula Margaritaki; Myrto Charitaki; Sune M. Christensen; Nikos S. Hatzakis; Kelly Velonia

doi:10.1039/d4py00407h

Oxygen-tolerant, eosin Y mediated synthesis of protein-polymer biohybrids and protein-coated polymer nanoparticles

Errika Voutyritsa, Thomai Lazou, Jonida Bushi, Stavroula Margaritaki, Myrto Charitaki, Sune M. Christensen, Nikos S. Hatzakis, Kelly Velonia^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

1 Citation (Scopus)

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Abstract

To avoid metal catalysts that are commonly used in conventional approaches for the synthesis of protein-polymer conjugates, an eosin Y/TEMED mediated, photoinduced polymerization of vinyl monomers was optimized. This oxygen tolerant, photoinduced approach allowed the grafting of a series of hydrophobic, hydrophilic and responsive polymers with quantitative protein macroinitiator consumption. CALB bioconjugates were also synthesized and found to retain part of the parent protein activity for extended periods of time. Notably, when BSA was used in the absence of an initiator, protein-coated nanoparticles were shown to form during emulsion polymerization.

Original language	English
Journal	Polymer Chemistry
Volume	15
Issue number	32
Pages (from-to)	3246-3255
Number of pages	10
ISSN	1759-9954
DOIs	https://doi.org/10.1039/d4py00407h
Publication status	Published - 2024

Bibliographical note

Funding Information:
We acknowledge Jeannette de Sparra Lundin, Gustav Hammerich Hansen and Christian Isak J\u00F8rgensen for assisting with CalB expression, purification, and characterization. The authors acknowledge the Hellenic Foundation for Research and Innovation (HFRI) E\u039BI\u0394EK program for funding under the 4th Call for HFRI PhD Fellowships (Fellowship Number: 11118). The authors acknowledge funding from the Independent Research Fund Denmark (1127-00432B), Villum Foundation (40801) and the NNF Challenge Center for Optimised Oligo Escape and Control of Disease (NNF23OC0081287).

Publisher Copyright:
© 2024 The Royal Society of Chemistry.

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10.1039/d4py00407hLicence: CC BY-NC

FulltextFinal published version, 1.85 MBLicence: CC BY-NC

Cite this

Oxygen-tolerant, eosin Y mediated synthesis of protein-polymer biohybrids and protein-coated polymer nanoparticles. / Voutyritsa, Errika; Lazou, Thomai; Bushi, Jonida; Margaritaki, Stavroula; Charitaki, Myrto; Christensen, Sune M.; Hatzakis, Nikos S.; Velonia, Kelly.

In: Polymer Chemistry, Vol. 15, No. 32, 2024, p. 3246-3255 .

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Oxygen-tolerant, eosin Y mediated synthesis of protein-polymer biohybrids and protein-coated polymer nanoparticles",

abstract = "To avoid metal catalysts that are commonly used in conventional approaches for the synthesis of protein-polymer conjugates, an eosin Y/TEMED mediated, photoinduced polymerization of vinyl monomers was optimized. This oxygen tolerant, photoinduced approach allowed the grafting of a series of hydrophobic, hydrophilic and responsive polymers with quantitative protein macroinitiator consumption. CALB bioconjugates were also synthesized and found to retain part of the parent protein activity for extended periods of time. Notably, when BSA was used in the absence of an initiator, protein-coated nanoparticles were shown to form during emulsion polymerization.",

author = "Errika Voutyritsa and Thomai Lazou and Jonida Bushi and Stavroula Margaritaki and Myrto Charitaki and Christensen, {Sune M.} and Hatzakis, {Nikos S.} and Kelly Velonia",

note = "Funding Information: We acknowledge Jeannette de Sparra Lundin, Gustav Hammerich Hansen and Christian Isak J\u00F8rgensen for assisting with CalB expression, purification, and characterization. The authors acknowledge the Hellenic Foundation for Research and Innovation (HFRI) E\u039BI\u0394EK program for funding under the 4th Call for HFRI PhD Fellowships (Fellowship Number: 11118). The authors acknowledge funding from the Independent Research Fund Denmark (1127-00432B), Villum Foundation (40801) and the NNF Challenge Center for Optimised Oligo Escape and Control of Disease (NNF23OC0081287). Publisher Copyright: {\textcopyright} 2024 The Royal Society of Chemistry.",

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AU - Voutyritsa, Errika

AU - Lazou, Thomai

AU - Bushi, Jonida

AU - Margaritaki, Stavroula

AU - Charitaki, Myrto

AU - Christensen, Sune M.

AU - Hatzakis, Nikos S.

AU - Velonia, Kelly

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PY - 2024

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N2 - To avoid metal catalysts that are commonly used in conventional approaches for the synthesis of protein-polymer conjugates, an eosin Y/TEMED mediated, photoinduced polymerization of vinyl monomers was optimized. This oxygen tolerant, photoinduced approach allowed the grafting of a series of hydrophobic, hydrophilic and responsive polymers with quantitative protein macroinitiator consumption. CALB bioconjugates were also synthesized and found to retain part of the parent protein activity for extended periods of time. Notably, when BSA was used in the absence of an initiator, protein-coated nanoparticles were shown to form during emulsion polymerization.

AB - To avoid metal catalysts that are commonly used in conventional approaches for the synthesis of protein-polymer conjugates, an eosin Y/TEMED mediated, photoinduced polymerization of vinyl monomers was optimized. This oxygen tolerant, photoinduced approach allowed the grafting of a series of hydrophobic, hydrophilic and responsive polymers with quantitative protein macroinitiator consumption. CALB bioconjugates were also synthesized and found to retain part of the parent protein activity for extended periods of time. Notably, when BSA was used in the absence of an initiator, protein-coated nanoparticles were shown to form during emulsion polymerization.

U2 - 10.1039/d4py00407h

DO - 10.1039/d4py00407h

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