PER1 Oscillation in Rat Parathyroid Hormone and Calcitonin Producing Cells

Birgitte Georg*, Henrik L. Jørgensen, Jens Hannibal

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Many endocrine glands exhibit circadian rhythmicity, but the interplay between the central circadian clock in the suprachiasmatic nucleus (SCN), the peripheral endocrine clock, and hormones is sparsely understood. We therefore studied the cellular localizations of the clock protein PER1, parathyroid hormone (PTH) and calcitonin (CT) in the parathyroid and thyroid glands, respectively. Thyroid glands, including the parathyroids, were dissected at different time-points from rats housed in 12 h:12 h light–darkness cycles, and were double-immunostained for PER1 and PTH or CT. Sera were analyzed for PTH, CT, phosphate, and calcium. In both glands, PER1 expression peaked late at night, while limited staining was seen during the daytime. High-resolution microscopy revealed cytosolic PER1 at zeitgeber time (ZT)12, and nucleic staining at ZT24 in both PTH and CT cells. PTH peaked at Z12–ZT16, while neither CT staining nor serum CT oscillated during the daily cycle. Serum PTH was significantly higher at ZT12 than ZT24, but only phosphate was found to exhibit significant diurnal oscillation. The staining of the calcium-sensitive receptor (CSR) did not demonstrate circadian oscillation. In conclusion, PER1 expression peaked late at night/early in the morning in hormone-producing cells of both the thyroid and parathyroid glands. In the parathyroids, this was preceded by a PTH peak, while neither CT nor CRS were found to oscillate.

Original languageEnglish
Article number13006
JournalInternational Journal of Molecular Sciences
Volume25
Issue number23
Number of pages13
ISSN1661-6596
DOIs
Publication statusPublished - 2024

Bibliographical note

Publisher Copyright:
© 2024 by the authors.

Keywords

  • calcitonin
  • parathyroid hormone
  • PER1
  • rat
  • thyroid gland

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