Phenotypic Characterization and Comparative Genomic Analysis of Novel Salmonella Bacteriophages Isolated from a Tropical Rainforest

Prasanna Mutusamy, Kirnpal Kaur Banga Singh, Lee Su Yin, Bent Petersen, Thomas Sicheritz-Ponten, Martha R. J. Clokie, Stella Loke, Andrew Millard, Sivachandran Parimannan, Heera Rajandas*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Salmonella infections across the globe are becoming more challenging to control due to the emergence of multidrug-resistant (MDR) strains. Lytic phages may be suitable alternatives for treating these multidrug-resistant Salmonella infections. Most Salmonella phages to date were collected from human-impacted environments. To further explore the Salmonella phage space, and to potentially identify phages with novel characteristics, we characterized Salmonella-specific phages isolated from the Penang National Park, a conserved rainforest. Four phages with a broad lytic spectrum (kills >5 Salmonella serovars) were further characterized; they have isometric heads and cone-shaped tails, and genomes of similar to 39,900 bp, encoding 49 CDSs. As the genomes share a

Original languageEnglish
Article number3678
JournalInternational Journal of Molecular Sciences
Volume24
Issue number4
Number of pages15
ISSN1661-6596
DOIs
Publication statusPublished - 2023

Keywords

  • Salmonella
  • bacteriophages
  • tropical rainforest
  • comparative genomics
  • HOST-RANGE
  • RESISTANCE
  • ALIGNMENT

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