TY - JOUR
T1 - PKC and AMPK regulation of Kv1.5 potassium channels
AU - Andersen, Martin Nybo
AU - Skibsbye, Lasse
AU - Tang, Chuyi
AU - Petersen, Frederic
AU - MacAulay, Nanna
AU - Rasmussen, Hanne Borger
AU - Jespersen, Thomas
PY - 2015
Y1 - 2015
N2 - The voltage-gated Kv1.5 potassium channel, conducting the ultra-rapid rectifier K(+) current (IKur), is regulated through several pathways. Here we investigate if Kv1.5 surface expression is controlled by the 2 kinases PKC and AMPK, using Xenopus oocytes, MDCK cells and atrial derived HL-1 cells. By confocal microscopy combined with electrophysiology we demonstrate that PKC activation reduces Kv1.5 current, through a decrease in membrane expressed channels. AMPK activation was found to decrease the membrane expression in MDCK cells, but not in HL-1 cells and was furthermore shown to be dependent on co-expression of Nedd4-2 in Xenopus oocytes. These results indicate that Kv1.5 channels are regulated by both kinases, although through different molecular mechanisms in different cell systems.
AB - The voltage-gated Kv1.5 potassium channel, conducting the ultra-rapid rectifier K(+) current (IKur), is regulated through several pathways. Here we investigate if Kv1.5 surface expression is controlled by the 2 kinases PKC and AMPK, using Xenopus oocytes, MDCK cells and atrial derived HL-1 cells. By confocal microscopy combined with electrophysiology we demonstrate that PKC activation reduces Kv1.5 current, through a decrease in membrane expressed channels. AMPK activation was found to decrease the membrane expression in MDCK cells, but not in HL-1 cells and was furthermore shown to be dependent on co-expression of Nedd4-2 in Xenopus oocytes. These results indicate that Kv1.5 channels are regulated by both kinases, although through different molecular mechanisms in different cell systems.
U2 - 10.1080/19336950.2015.1036205
DO - 10.1080/19336950.2015.1036205
M3 - Letter
C2 - 26043299
VL - 9
SP - 121
EP - 128
JO - Channels
JF - Channels
SN - 1933-6950
IS - 3
ER -