Polymorphism of Sakini Chicken Population From Different Locations/Altitudes of Nepal Using Randomly Amplified Polymorphic Dna Markers

Neena Amatya  Gorkhali; Rajan Dhakal; Saroj Sapkota; Prashanna Koirala; Bhoj Raj  Pokhrel; Mana Raj Kolachhapati; Neerajan Bhattarai

doi:10.3329/sja.v18i2.51113

Polymorphism of Sakini Chicken Population From Different Locations/Altitudes of Nepal Using Randomly Amplified Polymorphic Dna Markers

Neena Amatya Gorkhali, Rajan Dhakal, Saroj Sapkota, Prashanna Koirala, Bhoj Raj Pokhrel, Mana Raj Kolachhapati, Neerajan Bhattarai^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A study was conducted to evaluate genetic polymorphism in three Sakini chicken populations using random amplification of polymorphic DNA (RAPD) with seven highly polymorphic primers. All populations showed polymorphism with these primers that generates 59 different bands with an average of 8.4 bands per primer with 78.6% polymorphism nature. Primer OPA-16 produced the highest number of polymorphism bands 47 % and the lowest number of bands was produced by the OPA-05 primer 24 %. Differences for genetic distance (D) among populations were significant (P

Original language	English
Journal	SAARC Journal of Agriculture
Volume	18
Issue number	2
Pages (from-to)	115–124
Number of pages	10
DOIs	https://doi.org/10.3329/sja.v18i2.51113
Publication status	Published - 2021
Externally published	Yes

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title = "Polymorphism of Sakini Chicken Population From Different Locations/Altitudes of Nepal Using Randomly Amplified Polymorphic Dna Markers",

abstract = "A study was conducted to evaluate genetic polymorphism in three Sakini chicken populations using random amplification of polymorphic DNA (RAPD) with seven highly polymorphic primers. All populations showed polymorphism with these primers that generates 59 different bands with an average of 8.4 bands per primer with 78.6% polymorphism nature. Primer OPA-16 produced the highest number of polymorphism bands 47 % and the lowest number of bands was produced by the OPA-05 primer 24 %. Differences for genetic distance (D) among populations were significant (P",

author = "Gorkhali, {Neena Amatya} and Rajan Dhakal and Saroj Sapkota and Prashanna Koirala and Pokhrel, {Bhoj Raj} and Kolachhapati, {Mana Raj} and Neerajan Bhattarai",

year = "2021",

doi = "10.3329/sja.v18i2.51113",

language = "English",

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pages = "115–124",

journal = "SAARC Journal of Agriculture",

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T1 - Polymorphism of Sakini Chicken Population From Different Locations/Altitudes of Nepal Using Randomly Amplified Polymorphic Dna Markers

AU - Gorkhali, Neena Amatya

AU - Dhakal, Rajan

AU - Sapkota, Saroj

AU - Koirala, Prashanna

AU - Pokhrel, Bhoj Raj

AU - Kolachhapati, Mana Raj

AU - Bhattarai, Neerajan

PY - 2021

Y1 - 2021

N2 - A study was conducted to evaluate genetic polymorphism in three Sakini chicken populations using random amplification of polymorphic DNA (RAPD) with seven highly polymorphic primers. All populations showed polymorphism with these primers that generates 59 different bands with an average of 8.4 bands per primer with 78.6% polymorphism nature. Primer OPA-16 produced the highest number of polymorphism bands 47 % and the lowest number of bands was produced by the OPA-05 primer 24 %. Differences for genetic distance (D) among populations were significant (P

AB - A study was conducted to evaluate genetic polymorphism in three Sakini chicken populations using random amplification of polymorphic DNA (RAPD) with seven highly polymorphic primers. All populations showed polymorphism with these primers that generates 59 different bands with an average of 8.4 bands per primer with 78.6% polymorphism nature. Primer OPA-16 produced the highest number of polymorphism bands 47 % and the lowest number of bands was produced by the OPA-05 primer 24 %. Differences for genetic distance (D) among populations were significant (P

U2 - 10.3329/sja.v18i2.51113

DO - 10.3329/sja.v18i2.51113

M3 - Journal article

VL - 18

SP - 115

EP - 124

JO - SAARC Journal of Agriculture

JF - SAARC Journal of Agriculture

IS - 2

ER -