Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene

Helle Brander Eriksen; Andreas Petersen; Michael Pedersen; Søren Overballe-Petersen; Anders Rhod Larsen; Barbara Juliane Holzknecht

doi:10.1007/s10096-021-04344-w

Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene

Helle Brander Eriksen^*, Andreas Petersen, Michael Pedersen, Søren Overballe-Petersen, Anders Rhod Larsen, Barbara Juliane Holzknecht

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We describe a case of recurrent catheter-related blood stream infections (BSI) with Staphylococcusaureus, in which the first isolate tested susceptible to penicillin, while subsequent isolates were resistant. Phenotypic susceptibility correlated with the absence/presence of the blaZ gene. The in vitro stability of penicillin resistance was investigated by subculturing single colonies. In two out of five colonies, phenotypical resistance was lost after a single subculture, which correlated with loss of the blaZ gene. This in vitro phenomenon probably resulted in a very major error in the microbiology report of the first BSI, where penicillin had been recommended as treatment.

Original language	English
Journal	European Journal of Clinical Microbiology and Infectious Diseases
Volume	41
Issue number	1
Pages (from-to)	163-167
Number of pages	5
ISSN	0934-9723
DOIs	https://doi.org/10.1007/s10096-021-04344-w
Publication status	Published - 2022

Bibliographical note

Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

blaZ
In vitro susceptibility testing
PSSA PRSA
Staphylococcus aureus

Access to Document

10.1007/s10096-021-04344-w

Cite this

Eriksen, H. B., Petersen, A., Pedersen, M., Overballe-Petersen, S., Larsen, A. R., & Holzknecht, B. J. (2022). Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene. European Journal of Clinical Microbiology and Infectious Diseases, 41(1), 163-167. https://doi.org/10.1007/s10096-021-04344-w

Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene. / Eriksen, Helle Brander; Petersen, Andreas; Pedersen, Michael; Overballe-Petersen, Søren; Larsen, Anders Rhod; Holzknecht, Barbara Juliane.

In: European Journal of Clinical Microbiology and Infectious Diseases, Vol. 41, No. 1, 2022, p. 163-167.

Research output: Contribution to journal › Journal article › Research › peer-review

Eriksen, HB, Petersen, A, Pedersen, M, Overballe-Petersen, S, Larsen, AR & Holzknecht, BJ 2022, 'Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene', European Journal of Clinical Microbiology and Infectious Diseases, vol. 41, no. 1, pp. 163-167. https://doi.org/10.1007/s10096-021-04344-w

Eriksen, Helle Brander ; Petersen, Andreas ; Pedersen, Michael ; Overballe-Petersen, Søren ; Larsen, Anders Rhod ; Holzknecht, Barbara Juliane. / Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene. In: European Journal of Clinical Microbiology and Infectious Diseases. 2022 ; Vol. 41, No. 1. pp. 163-167.

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