Potential targets for glucagon-like peptide 2 (GLP-2) in the rat: distribution and binding of i.v. injected (125)I-GLP-2

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Abstract

Glucagon-like peptide 2 (GLP-2) is a 33-amino acid (1-33) intestinotrophic peptide. In this study, the distribution and binding of i.v. injected radiolabeled GLP-2 (1-33) were investigated in rats using autoradiography in order to target possible binding sites. The major part of (125)I-GLP-2 (1-33) was distributed to kidneys, liver, and the gastrointestinal tract. In the small intestine, a high density of grains was localized in the epithelium with a predominance in the luminal part of the villus. The saturability of (125)I-GLP-2 (1-33) was investigated by administration of excess amounts of non-radioactive GLP-2 (1-33) or the primary metabolite of GLP-2 degradation, GLP-2 (3-33). In the small intestine, (125)I-GLP-2 was displaced both by non-radioactive GLP-2 (1-33) and (3-33), suggesting that the uptake of GLP-2 (1-33) in the small intestine is receptor-specific and that the metabolite GLP-2 (3-33) may interact with the GLP-2 receptor.
Original languageEnglish
JournalPeptides
Volume21
Issue number10
Pages (from-to)1511-7
Number of pages7
ISSN0196-9781
Publication statusPublished - Oct 2000

Keywords

  • Animals
  • Autoradiography
  • Binding, Competitive
  • Epithelium
  • Female
  • Glucagon-Like Peptide 2
  • Glucagon-Like Peptides
  • Injections, Intravenous
  • Intestine, Small
  • Iodine Radioisotopes
  • Kidney
  • Liver
  • Peptides
  • Protein Binding
  • Radioimmunoassay
  • Rats
  • Rats, Wistar
  • Receptors, Glucagon
  • Substrate Specificity

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