Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR

Mathilde Gondard; Lorraine Michelet; Athinna Nisavanh; Elodie Devillers; Sabine Delannoy; Patrick Fach; Anna Aspan; Karin Ullman; Jan Chirico; Bernd Hoffmann; Fimme Jan van der Wal; Aline de Koeijer; Conny van Solt-Smits; Seta Jahfari; Hein Sprong; Karen L Mansfield; Anthony R Fooks; Kirstine Klitgaard; Rene Bødker; Sara Moutailler

doi:10.1093/femspd/fty083

Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR

Mathilde Gondard, Lorraine Michelet, Athinna Nisavanh, Elodie Devillers, Sabine Delannoy, Patrick Fach, Anna Aspan, Karin Ullman, Jan Chirico, Bernd Hoffmann, Fimme Jan van der Wal, Aline de Koeijer, Conny van Solt-Smits, Seta Jahfari, Hein Sprong, Karen L Mansfield, Anthony R Fooks, Kirstine Klitgaard, Rene Bødker, Sara Moutailler^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

34 Citations (Scopus)

21 Downloads (Pure)

Abstract

Ticks are one of the principal arthropod vectors of human and animal infectious diseases. Whereas the prevalence of tick-borne encephalitis virus in ticks in Europe is well studied, there is less information available on the prevalence of the other tick-borne viruses (TBVs) existing worldwide. The aim of this study was to improve the epidemiological survey tools of TBVs by the development of an efficient high-throughput test to screen a wide range of viruses in ticks.In this study, we developed a new high-throughput virus-detection assay based on parallel real-time PCRs on a microfluidic system, and used it to perform a large scale epidemiological survey screening for the presence of 21 TBVs in 18,135 nymphs of I. ricinus collected from five European countries. This extensive investigation has (i) evaluated the prevalence of four viruses present in the collected ticks, (ii) allowed the identification of viruses in regions where they were previously undetected.In conclusion, we have demonstrated the capabilities of this new screening method that allows the detection of numerous TBVs in a large number of ticks. This tool represents a powerful and rapid system for TBVs surveillance in Europe and could be easily customized to assess viral emergence.

Original language	English
Article number	fty083
Journal	Pathogens and Disease
Volume	76
Issue number	1
Number of pages	12
ISSN	2049-632X
DOIs	https://doi.org/10.1093/femspd/fty083
Publication status	Published - 2018
Externally published	Yes

Access to Document

10.1093/femspd/fty083

Fty083Accepted author manuscript, 1.52 MB
10Final published version, 1.89 MB

Cite this

Gondard, M., Michelet, L., Nisavanh, A., Devillers, E., Delannoy, S., Fach, P., Aspan, A., Ullman, K., Chirico, J., Hoffmann, B., van der Wal, F. J., de Koeijer, A., van Solt-Smits, C., Jahfari, S., Sprong, H., Mansfield, K. L., Fooks, A. R., Klitgaard, K., Bødker, R., & Moutailler, S. (2018). Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR. Pathogens and Disease, 76(1), [fty083]. https://doi.org/10.1093/femspd/fty083

Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR. / Gondard, Mathilde; Michelet, Lorraine; Nisavanh, Athinna; Devillers, Elodie; Delannoy, Sabine; Fach, Patrick; Aspan, Anna; Ullman, Karin; Chirico, Jan; Hoffmann, Bernd; van der Wal, Fimme Jan; de Koeijer, Aline; van Solt-Smits, Conny; Jahfari, Seta; Sprong, Hein; Mansfield, Karen L; Fooks, Anthony R; Klitgaard, Kirstine; Bødker, Rene; Moutailler, Sara.

In: Pathogens and Disease, Vol. 76, No. 1, fty083, 2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Gondard, M, Michelet, L, Nisavanh, A, Devillers, E, Delannoy, S, Fach, P, Aspan, A, Ullman, K, Chirico, J, Hoffmann, B, van der Wal, FJ, de Koeijer, A, van Solt-Smits, C, Jahfari, S, Sprong, H, Mansfield, KL, Fooks, AR, Klitgaard, K, Bødker, R & Moutailler, S 2018, 'Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR', Pathogens and Disease, vol. 76, no. 1, fty083. https://doi.org/10.1093/femspd/fty083

Gondard, Mathilde ; Michelet, Lorraine ; Nisavanh, Athinna ; Devillers, Elodie ; Delannoy, Sabine ; Fach, Patrick ; Aspan, Anna ; Ullman, Karin ; Chirico, Jan ; Hoffmann, Bernd ; van der Wal, Fimme Jan ; de Koeijer, Aline ; van Solt-Smits, Conny ; Jahfari, Seta ; Sprong, Hein ; Mansfield, Karen L ; Fooks, Anthony R ; Klitgaard, Kirstine ; Bødker, Rene ; Moutailler, Sara. / Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR. In: Pathogens and Disease. 2018 ; Vol. 76, No. 1.

@article{931881e0bb184d56bceedf2f12893500,

title = "Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR",

abstract = "Ticks are one of the principal arthropod vectors of human and animal infectious diseases. Whereas the prevalence of tick-borne encephalitis virus in ticks in Europe is well studied, there is less information available on the prevalence of the other tick-borne viruses (TBVs) existing worldwide. The aim of this study was to improve the epidemiological survey tools of TBVs by the development of an efficient high-throughput test to screen a wide range of viruses in ticks.In this study, we developed a new high-throughput virus-detection assay based on parallel real-time PCRs on a microfluidic system, and used it to perform a large scale epidemiological survey screening for the presence of 21 TBVs in 18,135 nymphs of I. ricinus collected from five European countries. This extensive investigation has (i) evaluated the prevalence of four viruses present in the collected ticks, (ii) allowed the identification of viruses in regions where they were previously undetected.In conclusion, we have demonstrated the capabilities of this new screening method that allows the detection of numerous TBVs in a large number of ticks. This tool represents a powerful and rapid system for TBVs surveillance in Europe and could be easily customized to assess viral emergence.",

author = "Mathilde Gondard and Lorraine Michelet and Athinna Nisavanh and Elodie Devillers and Sabine Delannoy and Patrick Fach and Anna Aspan and Karin Ullman and Jan Chirico and Bernd Hoffmann and {van der Wal}, {Fimme Jan} and {de Koeijer}, Aline and {van Solt-Smits}, Conny and Seta Jahfari and Hein Sprong and Mansfield, {Karen L} and Fooks, {Anthony R} and Kirstine Klitgaard and Rene B{\o}dker and Sara Moutailler",

year = "2018",

doi = "10.1093/femspd/fty083",

language = "English",

volume = "76",

journal = "FEMS Immunology and Medical Microbiology",

issn = "2049-632X",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR

AU - Gondard, Mathilde

AU - Michelet, Lorraine

AU - Nisavanh, Athinna

AU - Devillers, Elodie

AU - Delannoy, Sabine

AU - Fach, Patrick

AU - Aspan, Anna

AU - Ullman, Karin

AU - Chirico, Jan

AU - Hoffmann, Bernd

AU - van der Wal, Fimme Jan

AU - de Koeijer, Aline

AU - van Solt-Smits, Conny

AU - Jahfari, Seta

AU - Sprong, Hein

AU - Mansfield, Karen L

AU - Fooks, Anthony R

AU - Klitgaard, Kirstine

AU - Bødker, Rene

AU - Moutailler, Sara

PY - 2018

Y1 - 2018

N2 - Ticks are one of the principal arthropod vectors of human and animal infectious diseases. Whereas the prevalence of tick-borne encephalitis virus in ticks in Europe is well studied, there is less information available on the prevalence of the other tick-borne viruses (TBVs) existing worldwide. The aim of this study was to improve the epidemiological survey tools of TBVs by the development of an efficient high-throughput test to screen a wide range of viruses in ticks.In this study, we developed a new high-throughput virus-detection assay based on parallel real-time PCRs on a microfluidic system, and used it to perform a large scale epidemiological survey screening for the presence of 21 TBVs in 18,135 nymphs of I. ricinus collected from five European countries. This extensive investigation has (i) evaluated the prevalence of four viruses present in the collected ticks, (ii) allowed the identification of viruses in regions where they were previously undetected.In conclusion, we have demonstrated the capabilities of this new screening method that allows the detection of numerous TBVs in a large number of ticks. This tool represents a powerful and rapid system for TBVs surveillance in Europe and could be easily customized to assess viral emergence.

AB - Ticks are one of the principal arthropod vectors of human and animal infectious diseases. Whereas the prevalence of tick-borne encephalitis virus in ticks in Europe is well studied, there is less information available on the prevalence of the other tick-borne viruses (TBVs) existing worldwide. The aim of this study was to improve the epidemiological survey tools of TBVs by the development of an efficient high-throughput test to screen a wide range of viruses in ticks.In this study, we developed a new high-throughput virus-detection assay based on parallel real-time PCRs on a microfluidic system, and used it to perform a large scale epidemiological survey screening for the presence of 21 TBVs in 18,135 nymphs of I. ricinus collected from five European countries. This extensive investigation has (i) evaluated the prevalence of four viruses present in the collected ticks, (ii) allowed the identification of viruses in regions where they were previously undetected.In conclusion, we have demonstrated the capabilities of this new screening method that allows the detection of numerous TBVs in a large number of ticks. This tool represents a powerful and rapid system for TBVs surveillance in Europe and could be easily customized to assess viral emergence.

U2 - 10.1093/femspd/fty083

DO - 10.1093/femspd/fty083

M3 - Journal article

C2 - 30423120

VL - 76

JO - FEMS Immunology and Medical Microbiology

JF - FEMS Immunology and Medical Microbiology

SN - 2049-632X

IS - 1

M1 - fty083

ER -