Primary structure and localization of a conserved immunogenic Plasmodium falciparum glutamate rich protein (GLURP) expressed in both the preerythrocytic and erythrocytic stages of the vertebrate life cycle

M B Borre, M Dziegiel, B Høgh, E Pedersen, K Rieneck, E Riley, J F Meis, M Aikawa, K Nakamura, M Harada

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Abstract

A gene coding for a 220-kDa glutamate rich protein (GLURP), an exoantigen of Plasmodium falciparum, was isolated and its nucleotide sequence was determined. The deduced amino acid sequence contains 2 repeat regions. The sequence of one of these was shown to be conserved among geographically dispersed isolates, and a fusion protein containing that sequence was able to stimulate B- and T-cells. Antibodies against GLURP stained erythrocytic stages of the parasite as well as the hepatic stage as detected by electron microscopy.
Original languageEnglish
JournalMolecular and Biochemical Parasitology
Volume49
Issue number1
Pages (from-to)119-31
Number of pages12
ISSN0166-6851
Publication statusPublished - 1991

Bibliographical note

Keywords: Amino Acid Sequence; Animals; Antigens, Protozoan; Base Sequence; Chromosome Mapping; DNA, Protozoan; Erythrocytes; Microscopy, Immunoelectron; Molecular Sequence Data; Plasmodium falciparum; Protozoan Proteins

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