Producing Tailocins from Phages Using Osmotic Shock and Benzalkonium Chloride

Cedric Woudstra, Lone Brøndsted*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

3 Citations (Scopus)

Abstract

In the light of the worldwide antimicrobial resistance crisis, new substitutes to antibiotics are urgently needed. Tailocins or phage tail-like bacteriocin particles, produced by bacteria for environmental competition, are a potential antimicrobial alternative to antibiotic treatment. Yet, the availability of characterized Tailocins is limited. We explored the possibility to produce new Tailocins from phage particles, using osmotic shock or chemical treatment by the ammonium quaternary compound benzalkonium chloride on Ackermannviridae phage S117 and using Straboviridae phage T4 as control. We report that phage S117 was resistant to such treatment, while successful production of Tailocins by osmotic shock was achieved for phage T4. Finally, chemical treatment with benzalkonium chloride was inefficient on phage S117 but successfully inactivated phage T4 without production of Tailocins. Further studies are needed to implement such treatments of phages for producing Tailocins with killing activity.

Original languageEnglish
JournalPHAGE: Therapy, Applications, and Research
Volume4
Issue number3
Pages (from-to)136-140
Number of pages5
ISSN2641-6530
DOIs
Publication statusPublished - 2023

Bibliographical note

Publisher Copyright:
© Copyright 2023, Mary Ann Liebert, Inc., publishers 2023.

Keywords

  • antimicrobial
  • Bacteriophage
  • head-less phage
  • osmotic shock
  • phage tail-like particle
  • quaternary compound
  • Tailocin

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