Production and purification of Plasmodium circumsporozoite protein in Lactococcus lactis

Mohammad Naghizadeh, Susheel K Singh, Jordan Plieskatt, Ebenezer Addo Ofori, Michael Theisen

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Malaria is a vector-borne disease caused by Plasmodium parasites of which Plasmodium falciparum contributed to an estimated 247 million cases worldwide in 2021 (WHO malaria report 2022). The P. falciparum Circumsporozoite protein (PfCSP) covers the surface of the sporozoite which is critical to cell invasion in the human host. PfCSP is the leading pre-erythrocytic vaccine candidate and forms the basis of the RTS'S (Mosquirix®) malaria vaccine. However, high-yield production of full-length PfCSP with proper folding has been challenging. Here, we describe expression and purification of full-length PfCSP (containing 4 NVDP and 38 NANP repeats) with proper conformation by a simple three-step procedure in the Lactococcus lactis expression system.

Original languageEnglish
Title of host publicationRecombinant Glycoproteins : Methods and Protocols
EditorsSteven B. Bradfute
Number of pages13
PublisherHumana Press
Publication date2024
Pages109-121
DOIs
Publication statusPublished - 2024
SeriesMethods in molecular biology (Clifton, N.J.)
Volume27521
ISSN1064-3745

Bibliographical note

© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Cite this