Profiling of B cells and their subsets by whole blood gene expression analysis versus flow cytometry in multiple sclerosis

Sahla El Mahdaoui*, Marina Rode von Essen, Marie Mathilde Hansen, Jeppe Romme Christensen, Finn Sellebjerg, Helle Bach Søndergaard

*Corresponding author for this work

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Abstract

We investigated if differentially expressed mRNA targets could be used as surrogate markers for circulating B cells and subsets. In paired blood samples from patients with untreated, anti-CD20-treated, fingolimod-treated, and natalizumab-treated multiple sclerosis, whole blood expression of CD19 correlated with B cell counts determined by flow cytometry, ROR1 with transitional B cells, TCL1A and ZNF727 with naïve B cells, NEXMIF with memory B cells and BCMA with plasmablasts. CD19 expression distinguished patients with B cell repletion and may be used as an alternative to flow cytometry, but NEXMIF was unsuitable for memory B cell monitoring in rituximab-treated patients.

Original languageEnglish
Article number105898
JournalMultiple Sclerosis and Related Disorders
Volume91
Number of pages9
ISSN2211-0348
DOIs
Publication statusPublished - 2024

Bibliographical note

Publisher Copyright:
© 2024

Keywords

  • Anti-CD20
  • Cell sorting
  • Extended interval dosing
  • Infusion
  • Microarray

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