Protein aggregation capture on microparticles enables multi-purpose proteomics sample preparation

Tanveer Singh Batth, Maxim A. X. Tollenaere, Patrick Leopold Rüther, Alba Gonzalez-Franquesa, Bhargav Saligram Prabhakar, Simon Holst Bekker-Jensen, Atul Shahaji Deshmukh, Jesper V. Olsen

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Abstract

Universal proteomics sample preparation is challenging due to the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the inherent instability of denatured proteins for non-specific immobilization on microparticles by protein aggregation capture. To demonstrate the general applicability of this mechanism, we analyzed phosphoproteomes, tissue proteomes, and interaction proteomes as well as dilute secretomes. The findings presents a practical, sensitive and cost-effective proteomics sample preparation method.

Original languageEnglish
JournalMolecular and Cellular Proteomics
Volume18
Issue number5
Pages (from-to)1027-1035
ISSN1535-9476
DOIs
Publication statusPublished - 2019

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