Proteoglycan Isolation and Analysis

Anne Woods; John R. Couchman

doi:10.1002/cpcb.59

Proteoglycan Isolation and Analysis

Anne Woods, John R. Couchman

Research output: Contribution to journal › Journal article › Research › peer-review

3 Citations (Scopus)

Abstract

Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.

Original language	English
Article number	e59
Journal	Current Protocols in Cell Biology
Volume	80
Issue number	1
Number of pages	21
ISSN	1934-2500
DOIs	https://doi.org/10.1002/cpcb.59
Publication status	Published - 2018

Access to Document

10.1002/cpcb.59

Cite this

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title = "Proteoglycan Isolation and Analysis",

abstract = "Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.",

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AB - Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.

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DO - 10.1002/cpcb.59

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